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Degradation of phytate in rice bran by local bacteria phytase

Mohd Yusoff, Noraini and Mohd. Salleh, Hamzah and Jamal, Parveen (2010) Degradation of phytate in rice bran by local bacteria phytase. Special Issue of Research Journal of Chemistry and Environment. pp. 361-365. ISSN 0972-0626

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Abstract

Phytate (myo-inositol hexakisphosphates), a phosphorylated derivative of myo-inositol, is widely distributed in plants, particularly in cereals and legumes, such as corn, soybean, wheat bran, rice bran, cotton seeds, rape seeds and soybean with a concentration range between 0.4% to 6.4%. Rice bran, a by-product of rice milling, is usually either discarded as a waste or used to feed poultry and livestock. In the present work, phytase was used to hydrolyze phytate to yield inorganic orthophosphate (Pi) and a series of lower phosphoric esters of myo-inositol and in some cases, free myo-inositol. Myo-inositol phosphate intermediates formed by the dephosphorylation of phytate (IP6) by wild type phytate-degrading enzymes were partially purified by a stepwise gradient on an anion exchanger. Two different phytate degrading enzymes, ASUIA 271 and ASUIA 30 were used to degrade the myo-inositol hexakisphosphate under optimized process conditions. Phosphate released from the myo-inositol phosphates and percentage yield of the myo-inositol phosphate produced were measured and recorded. ASUIA 30 gave higher yield of myo-inositol phosphates compared to ASUIA 271.

Item Type: Article (Journal)
Additional Information: 2937/16811
Uncontrolled Keywords: myo-inositol phosphate, rice bran, anion exchange chromatography
Subjects: T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Prof.Dr. Parveen Jamal
Date Deposited: 27 Jan 2012 11:39
Last Modified: 10 Feb 2012 14:59
URI: http://irep.iium.edu.my/id/eprint/16811

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