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Nano-structural analysis of fish collagen extracts for new process development

Jaswir, Irwandi and Monsur, Hammed Ademola and Mohd. Salleh, Hamzah (2011) Nano-structural analysis of fish collagen extracts for new process development. In: 2nd International Conference on Biotechnology Engineering (ICBioE 2011), 17-19 May 2011, The Legend Hotel, Kuala Lumpur.

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Abstract

Nano and micro imaging were used to evaluate effects of pretreatment solution on gelatin. Four types of pre-treatment solution were used during fish gelatin extraction in this study. They were acetic acid (A), acetic acid-NaCl (SA), acetic acid-NaOH (BA) and acetic acid-NaOH-NaCl (SBA). Different pattern was observed for gelatins pretreated differently. Results showed that each pretreatment gave different nano imaging patterns; A (fibril), SA (zig-zag cracks), BA (straight rods) and SBA (cross-linked rods). Cross-linked rods observed in SBA denote adequate removal of non-collagen content of the fish skin and increase its surface area. SBA is suggested as the best pretreatment for perch fish gelatin. Result of viscosity was also highest for sample SBA (0.02450.0001 pas), while viscosity for others are 0.01550.0002 pas, 0.01230.0001 pas, and 0.00250.0001 pas for samples BA, SA and A, respectively. SDS-PAGE analysis showed presence of α and β in sample SBA and BA.

Item Type: Conference or Workshop Item (Full Paper)
Additional Information: 5784/16744
Uncontrolled Keywords: component; fish collagen; nano imaging; micro imaging; SDS-PAGE analysis
Subjects: T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes: Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Fuziah Arifin
Date Deposited: 26 Jan 2012 15:03
Last Modified: 26 Jan 2012 15:03
URI: http://irep.iium.edu.my/id/eprint/16744

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