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Enzymatic hydrolysis of oil palm empty fruit bunch using immobilized cellulase enzyme

Mohammed Abd., Rasha and Al-Khatib, Ma An Fahmi Rashid and Alam, Md. Zahangir and Mohd. Salleh, Hamzah (2011) Enzymatic hydrolysis of oil palm empty fruit bunch using immobilized cellulase enzyme. In: 2nd International Conference on Biotechnology Engineering (ICBioE 2011), 17-19 May 2011, The Legend Hotel, Kuala Lumpur.

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Abstract

The rapid development and the economic growth of oil palm industry have exacerbated environmental pollution since it results in the large quantity of waste products produced from the oil extraction process. In many cases, however, low catalytic efficiency and instability of enzymes were considered as barriers for the development of large-scale operations and applications. In this study, hydrolysis of Oil Palm Empty Fruit Bunch to reducing sugar was achieved using cellulase enzyme immobilized on multi-wall carbon nanotubes. The hydrolysis evaluation was performed using free cellulase enzyme. The amount of reducing sugar concentration released during the reaction was determined using Dinitrosalicylic acid (DNS) assay. Using the immobilized form of the enzyme, it was found that the reducing sugar concentration was increased and the enzymatic hydrolysis of Oil Palm Empty Fruit Bunch was enhanced greatly over long time of hydrolysis.

Item Type: Conference or Workshop Item (Full Paper)
Additional Information: 5595/16740 (Proceeding of the 2nd International Conference on Biotechnology Engineering (ICBioE 2011))
Uncontrolled Keywords: component; Oil Palm Empty Fruit Bunch; enzymatic hydrolysis; cellulase enzyme; immobilized cellulase enzyme.
Subjects: T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Dr. Ma'an Alkhatib
Date Deposited: 27 Jan 2012 11:50
Last Modified: 27 Jan 2012 11:59
URI: http://irep.iium.edu.my/id/eprint/16740

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