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Isolation of fuxoxanthin and fatty analysis from Padina australis and cytotoxicity effect of fucoxanthin of human lung cancer (H1299) cell lines

Noviendri, Dedi and Jaswir, Irwandi and Mohd. Salleh, Hamzah and Taher, Muhammad and Miyashita, Kazuo (2011) Isolation of fuxoxanthin and fatty analysis from Padina australis and cytotoxicity effect of fucoxanthin of human lung cancer (H1299) cell lines. In: Proceedings of the 2nd International Conference on Biotechnology Engineering ICBioE'11, 17-19 May 2011, Kuala Lumpur.

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Abstract

Fucoxanthin has been successfully isolated from species of Malaysian brown seaweed, namely P. australis. The purity of the fucoxanthin is >98% as indicated by High Performance Liquid Chromatography analysis. This seaweed also contained a considerable amount of unsaturated fatty acids. There are thirteen fatty acids were detected with Gas Chromatography. However, in terms of fatty acid methyl ester (FAMEs) of eicosapentanoic acid (C20:5n-3), arachidonic acid (C20:4n-6), linoleic acid (C18:2n-6) and alpha-linolenic acid (C18:3n-3) contents from P.australis was found (2.06, 9.50, 6.37, and 2.83 %, respectively). For saturated fatty acids, palmitic acid (C16:0) was found to be the major fatty acid about 23.97%. Furthermore, data obtained from the MTT assay indicated that fucoxanthin reduced the viability of H1299 cell lines, showing an IC50 value was 2.45 mM.

Item Type: Conference or Workshop Item (Full Paper)
Additional Information: 5784/16360
Uncontrolled Keywords: Brown seaweed; P. australis, Fucoxanthin; Fatty acids; H1299 cell lines, Human lung cancer Introduction
Subjects: T Technology > TP Chemical technology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering
Kulliyyah of Engineering > Department of Biotechnology Engineering
Kulliyyah of Pharmacy
Kulliyyah of Pharmacy > Department of Pharmaceutical Technology
Depositing User: Mrs. Wan Salwati Wan Salleh
Date Deposited: 17 Jan 2012 13:11
Last Modified: 17 Jan 2012 13:11
URI: http://irep.iium.edu.my/id/eprint/16360

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