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Enhancement of polymerase chain reaction using graphene nano-flakes

Rasheed, Abdul Khaliq and Kafafy, Raed and Faris, Waleed Fekry and Mohd. Salleh, Hamzah (2011) Enhancement of polymerase chain reaction using graphene nano-flakes. In: 2nd International Conference on Biotechnology Engineering (ICBioE 2011), 17-19 May 2011, The Legend Hotel, Kuala Lumpur.

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Abstract

The excellent heat transfer properties of nanoparticles have potential applications in various fields including biology during the last two decades. Recently, the use of various nanoparticles in polymerase chain reaction (PCR) resulted in significant enhancement of its efficiency and specificity. In this research we have demonstrated the effects of a novel material, graphene nano-flakes on PCR. The rationale behind the use of graphene flakes is its unique physical and heat transfer properties. A number of experimental results including the effect of graphene flakes on denaturation of DNA and annealing step will also be discussed. The preliminary results clearly show that enhanced heat transfer effect of nano-flakes augment PCR yield and ultimately overall enhancement in reaction efficiency.

Item Type: Conference or Workshop Item (Full Paper)
Additional Information: 5259/16194
Uncontrolled Keywords: component; Graphene flakes, Nano-PCR, nanoscale heat transfer
Subjects: T Technology > TJ Mechanical engineering and machinery
T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Dr Raed Kafafy
Date Deposited: 02 Feb 2012 12:33
Last Modified: 20 Jul 2012 06:43
URI: http://irep.iium.edu.my/id/eprint/16194

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