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Evaluation of nutritional components by Plackett-Burman design for Penicillium citrinum lipase production using palm oil mill effluent

Salihu, Aliyu and Alam, Md. Zahangir and Abdulkarim, Mohd Ismail and Hamzah, Mohd. Salleh (2011) Evaluation of nutritional components by Plackett-Burman design for Penicillium citrinum lipase production using palm oil mill effluent. African Journal of Biotechnology, 10 (81). pp. 18704-18708. ISSN 1684–5315

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Abstract

A number of medium components influencing lipase production by Penicillium citrinum (ATCC 42799) were studied using palm oil mill effluent (POME) as the basal medium. The medium components (peptone, yeast extract, malt extract, NH4Cl, NaNO3, KH2PO4, CaCl2, MgSO4, olive oil and Tween-80) were analyzed in twelve experimental trials using Plackett–Burman (PB) design. The most significant components affecting lipase production were found to be Tween-80, peptone, yeast extract, malt extract and NaNO3 at p < 0.05. The results indicate the efficiency of using PB design for screening processes. However, optimal concentration of the significant components can be determined by further statistical analysis.

Item Type: Article (Journal)
Additional Information: 4157/14377
Uncontrolled Keywords: Lipase, Penicillium citrinum, Plackett–Burman design, palm oil mill effluent.
Subjects: T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Dr. Md. Zahangir Alam
Date Deposited: 09 Jan 2012 15:49
Last Modified: 09 Jan 2012 15:49
URI: http://irep.iium.edu.my/id/eprint/14377

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