IIUM Repository

Optimization of Newcastle disease virus production in T-flask

Arifin, Mohd Azmir and Mel, Maizirwan and Salim, Siti Hajar and Abdul Karim, Mohd Ismail and Hassan, Sharifah Syed (2011) Optimization of Newcastle disease virus production in T-flask. African Journal of Biotechnology, 10 (81). pp. 18816-18823. ISSN 1684–5315

[img] PDF (Optimization of Newcastle disease virus production in T-flask) - Published Version
Restricted to Repository staff only

Download (840kB) | Request a copy


Newcastle disease (ND) is regarded as one of the most important diseases in the poultry industry. Currently, vaccines for ND are produced by using embryonated chicken eggs, a method which has the disadvantages of being labour-intensive, time consuming and requires large area for the incubation of eggs. In the present study, the production of lentogenic Asplin F strain of Newcastle disease virus by using cell culture method was studied. Experiments were carried out in T-flasks to investigate the effects of serum concentration in the culture medium during virus replication phase and multiplicity of infection (MOI) on ND virus propagation in DF-1 cells. Results show that virus infectivity titre of 6.62 x 107 TCID50/ml was achieved when serum concentration of 0.5% and MOI of 20 was used

Item Type: Article (Journal)
Additional Information: 4085/13687
Uncontrolled Keywords: Newcastle disease virus, Asplin F strain, DF-1 cell, T-flask
Subjects: T Technology > TP Chemical technology > TP155 Chemical engineering
T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Dr Maizirwan Mel
Date Deposited: 04 Jan 2012 14:55
Last Modified: 04 Jan 2012 14:56
URI: http://irep.iium.edu.my/id/eprint/13687

Actions (login required)

View Item View Item


Downloads per month over past year