Optimization of Newcastle disease virus production in T-flask

Arifin, Mohd Azmir and Mel, Maizirwan and Salim, Siti Hajar and Abdul Karim, Mohd Ismail and Hassan, Sharifah Syed (2011) Optimization of Newcastle disease virus production in T-flask. African Journal of Biotechnology, 10 (81). pp. 18816-18823. ISSN 1684–5315

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Abstract

Newcastle disease (ND) is regarded as one of the most important diseases in the poultry industry. Currently, vaccines for ND are produced by using embryonated chicken eggs, a method which has the disadvantages of being labour-intensive, time consuming and requires large area for the incubation of eggs. In the present study, the production of lentogenic Asplin F strain of Newcastle disease virus by using cell culture method was studied. Experiments were carried out in T-flasks to investigate the effects of serum concentration in the culture medium during virus replication phase and multiplicity of infection (MOI) on ND virus propagation in DF-1 cells. Results show that virus infectivity titre of 6.62 x 107 TCID50/ml was achieved when serum concentration of 0.5% and MOI of 20 was used

Item Type:	Article (Journal)
Additional Information:	4085/13687
Uncontrolled Keywords:	Newcastle disease virus, Asplin F strain, DF-1 cell, T-flask
Subjects:	T Technology > TP Chemical technology > TP155 Chemical engineering T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Dr Maizirwan Mel
Date Deposited:	04 Jan 2012 14:55
Last Modified:	04 Jan 2012 14:56
URI:	http://irep.iium.edu.my/id/eprint/13687

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