Zainal Abidin, Nur Hannah and Bakhtiar, M. Taher and Darnis, Deny Susanti and Mohd Jailani, Muhammad Taufiq and Khotib, Junaidi (2025) Synthesis of silver nanoparticles from dicranopteris linearis extract: physicochemical, cytotoxic, and antimicrobial characterization. Journal of King Saud University - Science, 37 (8). pp. 1-6. ISSN 1018-3647 E-ISSN 2213-686X
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Abstract
Extracts from plants are rich in phytochemicals that can be used as biogenic reducing agents for the synthesis of Ag (silver) nanoparticles (AgNPs). In this research, we synthesized AgNPs using Dicranopteris linearis leaves extract and assessed their cytotoxicity and antimicrobial activities. The aqueous extract of D. linearis (DL) leaves was analyzed using liquid chromatography-mass spectrometry (LC-MS). The extracts served as reducing agents for silver nitrate, producing AgNPs-DL. The nanoparticles were characterized by UV-visible spectrophotometry, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and dynamic light scattering (DLS), and cytotoxicity was assessed against human breast adenocarcinoma cells line (MCF-7) using the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. Antimicrobial activity was tested against four bacteria, such as Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, and Staphylococcus aureus, using the disc diffusion method. AgNPs-DL were successfully synthesized from D. linearis leaves extract. LC-MS/QTOF analysis revealed a diverse phytochemical profile, supporting the extract’s role in nanoparticle synthesis. SEM analysis showed predominantly spherical formation of AgNPs with size of 279.3 nm in average. Cytotoxicity assay indicated low toxicity against MCF-7 cells. AgNPs-DL also demonstrated inhibitory activity against E. coli, P. aeruginosa, B. subtilis, and S. aureus. Phytochemicals in D. linearis leaves extract contribute to stabilization and reduction of silver ions, resulting in AgNPs with distinct physicochemical properties and notable bioactivity
| Item Type: | Article (Journal) |
|---|---|
| Uncontrolled Keywords: | Antimicrobial activity, Biogenic synthesis, Cytotoxicity, Gleichenia linearis, Resam |
| Subjects: | R Medicine > RS Pharmacy and materia medica > RS192 Materia Medica-Pharmaceutical Technology |
| Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): | Kulliyyah of Pharmacy Kulliyyah of Pharmacy > Department of Pharmaceutical Technology Kulliyyah of Pharmacy > Department of Pharmacy Practice Kulliyyah of Science Kulliyyah of Science > Department of Chemistry |
| Depositing User: | Dr Deny Susanti Darnis |
| Date Deposited: | 11 Nov 2025 09:49 |
| Last Modified: | 11 Nov 2025 09:49 |
| URI: | http://irep.iium.edu.my/id/eprint/124088 |
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