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Isolation of antibacterial compounds from dichloromethane fraction of Malaysian sponges Neopetrosiaexigua

Qaralleh, Haitham and Syed Osman Idid, Syed Zahir Idid and Saad, Shahbudin and Darnis, Deny Susanti (2010) Isolation of antibacterial compounds from dichloromethane fraction of Malaysian sponges Neopetrosiaexigua. In: International Conference on Advancement in Science and Technology (iCAST2010): The Science of Traditional Practices in Health and Disease, 26-29 Nov 2010, Kuantan, Malaysia.

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This study was carried out to isolate the active antibacterial compounds from marine sponge Neopetrosiaexigua. Using disc diffusion methodm methanol extract of N. exigua showed significant antibacterial activity (The inhibition zone against Staphylococcus aureus was 19.5 mm). sequesntially, liquid-liquid extraction was performed using solvenants with different polarity (n-hexane, carbon tetrachloride, dicholoromathane, n-butanol and water in order to isolate the active antibacterial compounds. Bassed on the biological guided fractionation results, dichloromathane and n-butanol fractions showed the hisgest antimicrobial activity. Purification of dichloromathane fraction was done by using SephadexLH-20, normal phase column chromatographyand reverse phase column chromatography to yeild three compounds (1-3). All compounds were tested for their antibacterial activity against Staphyloccus aureus. The results showed that 3 exhibited significant antibacterial activity. Research is still in progress to elucidate the structures of the islated compounds.

Item Type: Conference or Workshop Item (Poster)
Additional Information: 5641/12158
Subjects: Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Science > Department of Biomedical Science
Kulliyyah of Science > Institute of Oceanography and Maritime Studies
Depositing User: Dr Deny Susanti Darnis
Date Deposited: 17 Jan 2012 12:31
Last Modified: 17 Jan 2012 13:12
URI: http://irep.iium.edu.my/id/eprint/12158

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