Deer velvet antler water extract demonstrates non-cytotoxic effects and maintains viability of dental stem cells

Azami, Azalea Nur-Qistina and Mokhtar@Makhtar, Khairani Idah and Mustafa Al-Ahmad, Basma Ezzat and Mohd Kenali, Norzaiti and Sha'ban, Munirah and Ahmad, Azlina and Zainol, Ismail (2025) Deer velvet antler water extract demonstrates non-cytotoxic effects and maintains viability of dental stem cells. Journal of International Dental and Medical Research, 18 (1). pp. 26-33. E-ISSN 1309-100X

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Abstract

Deer velvet antler (DVA), widely used in traditional Asian medicine, is gaining research interest for its effects on bone health, tissue regeneration, and cancer. However, the fundamental analysis looking into the effects of DVA on stem cells; particularly derived from dental tissues, are limited. Hence, this research aims to determine the biological effects of DVA water extract on the viability of stem cells harvested from human exfoliated deciduous teeth (SHED). DVA water extract was subjected for small molecule compounds analysis using LC-MS/MS-QTOF. The effects of DVA water extract on SHED were assessed at concentration up to 200 μg/ml for cell viability using MTT assay, cell morphology using inverted microscope and proliferative activity described as population doubling time (PDT) using alamarBlue assay. Although insignificant, DVA water extract at 3.125 μg/ml produced the lowest PDT while maintaining the cell proliferation and viability. Overall, DVA water extract at concentration up to 200 μg/ml was not cytotoxic, maintained its proliferative effects and the morphology of SHED. The presence of detectable small molecules in water extract which corresponds to the multiple benefits of DVA might be responsible for the demonstrated observations.

Item Type:	Article (Journal)
Uncontrolled Keywords:	Velvet antler; dental stem cells; water extract, biological activity
Subjects:	R Medicine > RK Dentistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Dentistry > Department of Fundamental Dental and Medical Sciences Kulliyyah of Dentistry
Depositing User:	Assoc Prof Khairani Idah Mokhtar
Date Deposited:	09 Apr 2025 10:15
Last Modified:	09 Apr 2025 10:15
URI:	http://irep.iium.edu.my/id/eprint/120493

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