Association of rs8670 polymorphism in the MSX1 gene with non-syndromic cleft lip with or without cleft palate in Malay population

Rashid, Roslina and Ahmad Rajion, Zainul and Zilfalil, Alwi and Jaafar, Saidi (2024) Association of rs8670 polymorphism in the MSX1 gene with non-syndromic cleft lip with or without cleft palate in Malay population. Cureus, 16 (9). pp. 1-7. ISSN 2168-8184

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Abstract

Objective: This study aimed to investigate the association between variants present in the MSX1 gene and the risk of developing non-syndromic cleft lip with or without cleft palate (NSCL±P) among individuals of Malay ethnicity in Malaysia. Materials and methods: This case-control study involved 89 patients with NSCL±P and 100 healthy control subjects. Polymerase chain reaction (PCR) was performed on both exon 1 and exon 2 of the MSX1 gene using four pairs of primers. The amplification products were then subjected to denaturing high-pressure liquid chromatography for initial screening, and the presence of a heteroduplex peak was validated using direct sequencing analysis to detect the single-nucleotide polymorphism. Results: Five previously known variations (c.-36G>A, p.Ala30Ala, p.Ala34Gly, p.Gly110Gly, and rs8670: C>T) were detected within the MSX1 gene in both NSCL±P patients and controls. A significant association was found between the rs8670: C>T variant and NSCL±P (p = 0.017; OR: 0.368; 95% CI: 0.152 - 0.893), with this particular single-nucleotide polymorphism present in 20% (20) among controls and 7.9% (7) of the NSCL±P cases. Conclusions: Our data showed a lower incidence of the rs8670: C>T polymorphism among NSCL±P cases compared to control in this Malay population. However, since this variant is located in the 3’UTR, it could potentially impact the stability of MSX1 mRNA.

Item Type:	Article (Journal)
Additional Information:	9602/119189
Uncontrolled Keywords:	case control studies, genetic polymorphisms, genetic variant, msx1, cleft lip with or without cleft palate
Subjects:	R Medicine > RK Dentistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Dentistry Kulliyyah of Dentistry > Department of Oral Maxillofacial Surgery and Oral Diagnosis
Depositing User:	Muhammad Akmal Safiuddin Rosli
Date Deposited:	08 Feb 2025 17:29
Last Modified:	08 Feb 2025 17:30
URI:	http://irep.iium.edu.my/id/eprint/119189

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