Magnetic metal-organic frameworks as sensitive aptasensors for coronavirus spike protein

Malla, Pravanjan and Liu, Chi-Hsien and Wu, Wei-Chi and Nordin, Anis Nurashikin and Rath, Dharitri (2024) Magnetic metal-organic frameworks as sensitive aptasensors for coronavirus spike protein. Analytica Chimica Acta, 1309. pp. 1-10. ISSN 0003-2670 E-ISSN 1873-4324

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Abstract

Electrochemical biosensors, known for their low cost, sensitivity, selectivity, and miniaturization capabilities, are ideal for point-of-care devices. The magnetic metal-organic framework (MMOF), synthesized using the in-situ growth method, consists of ferric salt, magnetic nanoparticles, histidine, and benzene tetracarboxylic acid. MMOF was sequentially modified with aptamer-biotin and streptavidin-horseradish peroxidase, serving as a detector for spike protein and a transducer converting electrochemical signals using H2O2-hydroquinone on a screen-printed electrode. MMOF facilitates easy washing and homogeneous deposition on the working electrode with a magnet, enhancing sensitivity and reducing noise. The physical and electrochemical properties of the modified MMOFs were thoroughly characterized using various analytical techniques. The aptasensors' performance achieved a detection limit of 6 pM for voltammetry and 5.12 pM for impedance spectroscopy in human serum samples. This cost-effective, portable MMOF platform is suitable for rapid point-of-care testing for SARS-CoV-2 spike proteins

Item Type:	Article (Journal)
Uncontrolled Keywords:	Aptasensor Magnetic metal-organic framework Spike protein Coronavirus
Subjects:	T Technology > TK Electrical engineering. Electronics Nuclear engineering > TK7800 Electronics. Computer engineering. Computer hardware. Photoelectronic devices
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Electrical and Computer Engineering Kulliyyah of Engineering
Depositing User:	Dr. Anis Nurashikin Nordin
Date Deposited:	07 May 2024 09:41
Last Modified:	07 May 2024 10:21
URI:	http://irep.iium.edu.my/id/eprint/112089

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