Total phenolics contents and free radical scavenging capacity of piper sarmentosum (pucuk kaduk) extract

Mat Jusoh, Hanapi and Haron, Normah and Mohd Zohari, Sarah Haryati (2019) Total phenolics contents and free radical scavenging capacity of piper sarmentosum (pucuk kaduk) extract. International Journal of Allied Health Sciences, 3 (4). pp. 875-882. E-ISSN 2600-8491

PDF - Published Version Restricted to Registered users only Download (307kB) | Request a copy

Abstract

Introduction: Piper sarmentosum is one of the herbaceous plants that has been used as natural antioxidant to source to treat diseases. This study was conducted to determine the total phenolic contents (TPC) and free radical scavenging capacity in free and bound (soluble and insoluble) of P. sarmentosum. Methods: Free phenolic extract was acquired through direct methanol extraction while acidic and alkaline hydrolyses were adopted to release the bound phenolic acids. The TPC was determined by using Folin-Ciocalteu assay and is expressed as Gallic Acid equivalent (GAE) in miligrams per gram of extracts. The antioxidant scavenging capacity was determined by using DPPH (2, 2-diphenyl-1-picrylhydrazyl) assay. Results: Insoluble bound phenolic extract of P. sarmentosum showed the highest TPC value (1.54 ± 0.04 mg GAE/g DW) followed by soluble phenolic extract and free extract (1.13 ± 0.10 and 0.57 ± 0.06 mg GAE/g DW, respectively). The soluble phenolic fraction has expressed the highest free radical scavenging capacity (76.57± 4.12%) followed by insoluble (69.79± 2.33 %) and free extracts (58.15± 4.44 %). The IC50 values for free, soluble and insoluble bound phenolic were 24.05 ± 3.81, 16.17 ± 1.84 and 18.49 ± 1.92 mg/ml, respectively. Conclusions: The significant differences between all the extracts and antioxidant inhibition in this present study suggested that different forms (free and bound) of extracts did influence the radical scavenging capacity as a whole.

Item Type:	Article (Journal)
Subjects:	Q Science > QD Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences > Department of Nutrition Sciences Kulliyyah of Allied Health Sciences
Depositing User:	Dr Hanapi Mat Jusoh
Date Deposited:	08 Feb 2024 12:18
Last Modified:	08 Feb 2024 12:18
URI:	http://irep.iium.edu.my/id/eprint/110796

Actions (login required)

View Item