The antioxidant activity and induction of apoptotic cell death by Musa paradisiaca and Trigona sp. honey jelly in ORL115 and ORL188 cells

Anuar, Mohd Nur Nasyriq and Ibrahim, Muhammad and Zakaria, Nor Hafizah and Ichwan, Solachuddin Jauhari Arief and Md. Isa, Muhammad Lokman and Mat Alewi, Nur Aizura and Hagar, Abdullah and Abdul Majid, Fadzilah Adibah (2023) The antioxidant activity and induction of apoptotic cell death by Musa paradisiaca and Trigona sp. honey jelly in ORL115 and ORL188 cells. Malaysian Journal of Medical Sciences, 30 (1). pp. 82-91. ISSN 1394-195X E-ISSN 2180-4303

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Abstract

Background: Head and neck cancer patients usually need nutritional support due to difficulties in swallowing and chewing. Therefore, this study aimed to formulate Musa paradisiaca and Trigona sp. honey jelly (MTJ) as a convenient functional food. Methods: The antioxidant properties were analysed using 2,2′-diphenyl-1 picrylhydrazyl (DPPH), ferric reducing antioxidant potential (FRAP) and 2,2′-azinodi 3-ethylbenthiazolinesulfonate (ABTS) assays. Cytotoxicity was assayed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) test and the induction of apoptosis was observed via caspase-3/7 activity assay. The identification of phenolic compounds was done via ultra-high-performance-liquid chromatography coupled to mass spectrometer (UHPLC-MS/MS). Results: The antioxidant analysis exhibited: the half inhibitory concentration (IC50) of DPPH inhibition, 54.10 (SD = 4.51) µg/mL; the FRAP value, 30.07 (SD = 0.93) mM TEQ/100 g; and the ABTS value, 131.79 (SD = 8.73) mg TEQ/100 g. Cinnamic acid was the most abundant phenolic compound, followed by maleic acid and salicylic acid. The IC50 for ORL115 and ORL188 were 35.51 mg/mL and 43.54 mg/mL, respectively. The cells became rounded and dissymmetrical which reduced in number and size. The apoptotic cell death in ORL115 and ORL188 was deduced as caspase-3/7 activities that significantly increased (P < 0.05). Conclusion: The study evidenced that the antioxidant activity of MTJ could influence the induction of apoptosis in ORL115 and ORL188 in future investigations and verifications.

Item Type:	Article (Journal)
Uncontrolled Keywords:	antioxidant; apoptosis; head and neck squamous cell carcinoma; Musa paradisiaca; stingless bee
Subjects:	Q Science > Q Science (General) R Medicine > R Medicine (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences > Department of Nutrition Sciences Kulliyyah of Nursing > Department of Basic Medical Sciences for Nursing Kulliyyah of Allied Health Sciences Kulliyyah of Nursing Institute of Planetary Survival for Sustainable Well-Being (PLANETIIUM)
Depositing User:	Muhammad Ibrahim
Date Deposited:	18 Oct 2023 14:57
Last Modified:	18 Oct 2023 15:24
URI:	http://irep.iium.edu.my/id/eprint/107522

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