Optimizing the extraction of secondary metabolites from Ulva lactuca using response surface methodology

Shukor, Muhammad Idham and Darnis, Deny Susanti and Aminudin, Nurul Iman and Mohammad Noor, Normawaty and Bakhtiar, M. Taher (2023) Optimizing the extraction of secondary metabolites from Ulva lactuca using response surface methodology. In: International Conference on Natural Products 2023 (ICNP 2023), Kuala Lumpur. (Unpublished)

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Abstract

The various extraction of secondary metabolites from various biomass is a recent rising interest in an attempt to find natural alternatives for the chemical compounds cosmetical and pharmaceuticals industries. These extraction methods can be further modified with several factors to suit specific requirements. The present work aimed to screen and optimized the extraction of secondary metabolites from different drying methods (sun-dried, air-dried, oven-dried, and freeze-dried) of Ulva lactuca using orbital shaker-assisted extraction, conventional solvent extraction, and ultrasonic-assisted extraction. In this respect, a screening procedure using a Two-Level factorial design was employed to assess the effect of three different factors at two levels and their interactions on the concentration of secondary metabolites from U. lactuca. The studied factors were solute-to-solvent ratio, time of extraction, and temperature. The two levels chosen for extraction were solute-to-solvent ratio (1:20 to 1:50 g/mL), extraction time (20 to 60 mins), and temperature (30 to 70°C). N-hexane:acetone: methanol (2:1:1) was used as an extracting solvent. β-Carotene, fucoxanthin, and quercetin concentrations were set as responses for optimum output. Further analysis for optimization was conducted by using Central Composite Design. The results showed that all factors are dominant and significantly affect the extraction process. The optimum extraction parameters were solute-to-solvent ratio (1:31, 1:52, and 1:50 g/mL), extraction time (84, 87, and 75 mins), and temperature (32, 34, and 35°C) for β-carotene, fucoxanthin, and quercetin, respectively. Under these conditions, the maximum value for β-carotene was calculated as 0.168891 mg/g, fucoxanthin (0.1067 mg/g) and quercetin showed a maximum calculated value of 0.09733 mg/g with the desirability of 0.98178, 0.988 and 0.994, respectively. The present study indicates that U. lactuca can be used as a source of carotenoids and phenolic compounds that can be applied in cosmetical and pharmaceutical products as optimum extraction conditions.

Item Type:	Conference or Workshop Item (Slide Presentation)
Uncontrolled Keywords:	Ulva lactuca; β-Carotene; fucoxanthin; quercetin; factorial design
Subjects:	Q Science > QD Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Chemistry Kulliyyah of Pharmacy Kulliyyah of Pharmacy > Department of Pharmaceutical Technology Kulliyyah of Science Kulliyyah of Science > Department of Marine Science
Depositing User:	Dr Deny Susanti Darnis
Date Deposited:	21 Sep 2023 13:28
Last Modified:	21 Sep 2023 13:28
URI:	http://irep.iium.edu.my/id/eprint/106877

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