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Myo-inositol ameliorative effects on cryo-survival of post-thawed abnormal human sperms quality towards infertility care

Abdul Wahab, Azantee Yazmie and Ahmad Yusof, Zamri and Abdul Rahman, Suzanah and Md Isa, Muhammad Lokman and Mazlan, Muhammad Alif (2023) Myo-inositol ameliorative effects on cryo-survival of post-thawed abnormal human sperms quality towards infertility care. Journal of Reproductive Biotechnology and Fertility. ISSN 20589158

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Background Sperm cryopreservation usually results in a significant decrease in sperm quality after thawing. The deleterious effects of freeze-thaw on abnormal sperm samples are considerably more severe. Various supplemental agents have been studied to ameliorate the negative effects due to cryopreservation. Myo-Inositol is likely to positively affect fertility. Therefore, this study aims to evaluate the beneficial effect of Myo-inositol (MI) supplementation in sperm freezing media for abnormal sperm. Methods Semen samples were collected from 23 male subjects that have abnormal sperm quality. Semen analysis has been conducted before freezing (pre-freeze). The samples were frozen with only a freezing medium (control) or MI-supplemented freezing medium. Semen analysis was re-evaluated after post-thaw for both groups. GoldCyto test kits were used in both post-thaw samples to evaluate the DNA fragmentation. Results The total motility, Progressive motility, morphology, and viability mean values of the sperms frozen with the MI-supplemented medium are greater compared to the control group (p<0.05). The post-thaw sample with MI supplementation (39.8%) has a higher cryo-survival rate (CSR) compared to the control group (23.4%). Conclusion MI supplementation in cryopreserved abnormal sperm samples significantly enhanced the post-thaw sperm motility, morphology, and vitality, and potentially increase the sperm survival rate.

Item Type: Article (Journal)
Uncontrolled Keywords: Myo-inositol supplementation, sperm cryopreservation, DNA fragmentation, abnormal sperm quality, cryodamage
Subjects: Q Science > Q Science (General)
Q Science > QM Human anatomy
Q Science > QP Physiology
R Medicine > R Medicine (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011)
Kulliyyah of Nursing > Department of Basic Medical Sciences for Nursing
Institute of Planetary Survival for Sustainable Well-Being (PLANETIIUM)
Depositing User: Dr Muhammad lokman M Isad
Date Deposited: 11 Sep 2023 17:46
Last Modified: 11 Sep 2023 17:46
URI: http://irep.iium.edu.my/id/eprint/106514

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