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Media screening and optimization of lactic acid fermentation

Abdul Karim, Mohamed Ismail and Mel, Maizirwan and Jamal, Parveen and Mohamed Salleh, Mohamad Ramlan and Alamin, Noraini (2005) Media screening and optimization of lactic acid fermentation. In: 2nd International Conference on Chemical and Bioprocess Engineering (ICCBpE-SOMChE 2005), 8-10 Dec 2005, Kota Kinabalu, Sabah.

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Abstract

The screening and optimization of lactic acid production using bacteria L. rhamnosus were successfully carried out in shake flask experiment. Initially eleven variables were screened using Placket Burman Design and result indicated that, the main variables that effected were glucose and peptone. These two most significant variables were then optimized using the Central Composite Design. The correlation between those two variables was analyzed using Response Surface Methodology (RSM). The contour plot indicates that the interactions between these two variables are not much significantly different. The optimization and critical value indicate that the most ideal concentration of glucose and peptone for optimum production of lactic acid in shake flask is 9.80g/l and 9.98g/l, respectively. The maximum growth rate for these bacteria is 0.394 h-1 and the productivity is 0.630 g/g.h.

Item Type: Conference or Workshop Item (Full Paper)
Additional Information: 4684/10635
Subjects: T Technology > TP Chemical technology > TP248.13 Biotechnology
T Technology > TP Chemical technology > TP500 Fermentation industries. Beverages. Alcohol
Kulliyyahs/Centres/Divisions/Institutes: Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Prof.Dr. Parveen Jamal
Date Deposited: 17 May 2013 15:23
Last Modified: 17 May 2013 15:23
URI: http://irep.iium.edu.my/id/eprint/10635

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