Ahda, Mustofa and Jaswir, Irwandi and Subara, Deni
(2022)
Enhancement of physical properties of gelatin-based film by bovine serum albumin.
Carpathian Journal of Food Science & Technology.
ISSN 2066-6845
E-ISSN 2344-5459
Abstract
Biopolymer-based films are created from plant or animal materials such as
carbohydrates and proteins. In this study, a biopolymer-based film from
gelatin and Bovine Serum Albumin (BSA) was created to form a film
through cross-linking interaction of both biopolymers. The synthesized
biopolymer film was characterized using Differential Scanning Calorimetry
(DSC), Scanning Electron Microscopy (SEM), and Fourier Transform
Infrared (FTIR) as morphological evaluation. Besides, the encapsulation
efficiency of BSA-gelatin blend film was also evaluated. The coagulation
process was performed using acetone carried out at 35 oC for 4 hours under
stirring. The formed particles were separated from the solution using high
centrifugation around 12,000 rpm for 30 min and then kept at -80 oC.
Based on the FTIR spectra showed that BSA and gelatin have the same
functional group such as hydroxyl (OH) and Amina I, II, and III which
appear at 3278 cm-1
, 1633 cm-1
, 1542 cm-1
, and 1241 cm-1
, respectively.
After linkage reaction, the gelatin-BSA interaction created more strength
interaction that caused no vibration functional groups seen. It also increased
glass transition temperature where the resulted melting point of gelatinbased and 70% BSA/gelatin-based films are 152.2 and 260 °C, respectively.
The encapsulated BSA in the blend film reached above 90% in the
BSA/gelatin ratio of 70%.
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