Earlier denaturation of DNA by using novel ternary hybrid nanoparticles

Zayan, Jalal Mohammed and John, Akbar and Rasheed, Abdul Khaliq and Alallam, Batoul and Khalid, Mohammed and Ismail, Ahmad Faris and Nelson, Bryan Raveen and Salleh, Hamzah Mohd. (2022) Earlier denaturation of DNA by using novel ternary hybrid nanoparticles. IIUM Engineering Journal, 23 (2). pp. 237-245. ISSN 1511-758X E-ISSN 2289-7860

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Abstract

Two novel ternary hybrid nanoparticles (THNp) consisting of graphene oxide (GO) and reduced graphene oxides (rGO) were added to samples of DNA. The effect of the addition of nanoparticles on the thermal denaturation of DNA samples was studied by measuring the absorbance using a temperature-controlled Perkin Elmer UV spectrophotometer. Adding GO-TiO 2-Ag and rGO-TiO 2-Ag nanoparticles lowered the denaturation temperature of template DNA significantly. The nanoparticles affect the denaturation rate. The optimal GO-TiO 2-Ag and rGO-TiO 2-Ag concentrations were found to be 5× 10-2, which resulted in 86-and 180-folds augmentation of DNA denaturation (6.5 µg/mL), respectively, while it resulted in 2-and 7-folds augmentation of DNA denaturation (11.5 µg/mL), respectively, at temperature as low as 80 C. The results indicated that rGO-TiO 2-Ag nanoparticles exhibited significantly higher DNA denaturation enhancement than rGO-TiO 2-Ag nanoparticles, owing to their enhanced thermal conductivity effect. Therefore, these nanoparticles could help to get improved PCR yield, hence enable amplification to be performed for longer cycles by lowering the denaturation temperature

Item Type:	Article (Journal)
Uncontrolled Keywords:	DNA denaturation; polymerase chain reaction (PCR); nano-PCR; hybrid nanoparticles
Subjects:	T Technology > TP Chemical technology > TP155 Chemical engineering
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Mechanical Engineering Kulliyyah of Engineering
Depositing User:	Prof Dr Ahmad Faris Ismail
Date Deposited:	09 Jan 2023 15:47
Last Modified:	06 Jun 2023 13:38
URI:	http://irep.iium.edu.my/id/eprint/103068

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