IIUM Repository

In vitro α-glucosidase inhibitory activity of Mahkota Dewa fruit flesh using different extraction methods

Abdur Rashid, Mohammad and Sarker, Md. Zaidul Islam and Ahmed, Qamar Uddin and Ferdosh, Sahena and Helal Uddin, A.B.M. (2021) In vitro α-glucosidase inhibitory activity of Mahkota Dewa fruit flesh using different extraction methods. In: International conference of Intigrative Medicine, 17-19 August 2021, USM, Penang. (Unpublished)

PDF - Supplemental Material
Download (1MB) | Preview


Purpose: Before using any plant for medicinal purposes, the proper extraction method must be considered for the bioactive compounds, which can be either polar or non-polar or both, and its advantages and disadvantages, depending on the selection process. The aim of this research was to compare the α-glucosidase inhibitory activity of Mahkota dewa fruit flesh using conventional and non-conventional extraction techniques. Method: The extraction was performed by heat reflex (HR), supercritical fluid extraction (SFE), and subcritical carbon dioxide (Sub-CO2) extraction using different temperatures, pressure, solvent ratio, flow rate. The optimized extracts were analyzed for a-glucosidase inhibitory activity with Saccharomyces cerevisiae enzyme. Result: The yields and α-glucosidase inhibitory activity of the Mahkota dewa extracts were analyzed. The yield was obtained in the order of HR>SubCO2>SFE on the other hand the IC50 value of α- glucosidase inhibitory activity was estimated in the order of SubCO2> HR >SFE due to its mild operating temperature and relatively low-pressure condition. Because carbon dioxide is a non- polar solvent that prefers mostly non-polar compounds, adding a co- solvent increases its polarity and improves the ability to extract more polar compounds. Furthermore, after depressurization, carbon dioxide can be easily separated from the end product. As a result, SubCO2 is the most practical extraction method for achieving higher quality of extract. Conclusion: The composition and bioactivities of extracts of natural products were typically influenced by the extracting method, solvent, and temperature.

Item Type: Conference or Workshop Item (Invited Papers)
Subjects: Q Science > QD Chemistry
R Medicine > RM Therapeutics. Pharmacology
R Medicine > RS Pharmacy and materia medica
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Depositing User: Dr. A. B. M. Helal Uddin
Date Deposited: 02 Dec 2022 11:11
Last Modified: 02 Dec 2022 11:11
URI: http://irep.iium.edu.my/id/eprint/101334

Actions (login required)

View Item View Item


Downloads per month over past year