Mohamad, Anis Amirah and Abdul Majid, Fadzilah Adibah and Assaw, Suvic and Zainal Abidin, Fadhilah and Fadhlina, Anis and Aluru, Sudheer Kumar and Zainol, Siti Nurazwa and Ahmed, Najat Jabbar (2021) Anti-Inflammatory and oral toxicity assessment Of R-38TM: a traditional herbal supplement for arthritis. Jurnal Teknologi, 84 (1). pp. 249-262. E-ISSN 2180–3722
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Abstract
R-38TM is a traditional herbal supplement for treating arthritis-related conditions. High-performance liquid chromatography (HPLC) analysis was performed for identification and quantification of rosmarinic acid in the R-38TM water extract. The anti-arthritic potential of the R-38TM water extract was investigated by measuring the production of IL-6 and TNF-α in inflamed cells. Xanthine oxidase (XO) and cyclooxygenase-2 (COX-2) inhibition assays were also conducted. The cytotoxic effect of R-38TM water extract was investigated on HSF1184 cell line. Acute and subacute oral toxicity studies were conducted on female Sprague�Dawley (SD) rats. The rosmarinic acid was identified at 1.208 min (3.61 %w/w). The inflamed cells showed a decrease in the production of IL-6 (55.9%) and TNF-α (52.13%). The COX-2 and XO enzymes were moderately inhibited by R-38TM water extract. The cytotoxicity analysis showed no cytotoxic effect on the cell. The acute and subacute oral toxicity studies revealed no mortality and normal body weight at all doses. There were no significant differences (p > 0.05) in organ weight, hematological and biochemical parameters, and histology of liver and kidneys with the control group. In conclusion, R-38TM water extract exhibited no toxic effect orally and may possess potential therapeutic properties against pro-inflammatory diseases including arthritis.
| Item Type: | Article (Journal) |
|---|---|
| Uncontrolled Keywords: | Botanical medicine, R-38TM, anti-inflammatory, oral toxicity, rosmarinic acid, arthritis |
| Subjects: | Q Science > QD Chemistry R Medicine > RM Therapeutics. Pharmacology |
| Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): | Kulliyyah of Dentistry > Department of Fundamental Dental and Medical Sciences |
| Depositing User: | Dr. Anis Fadhlina |
| Date Deposited: | 21 Nov 2022 08:36 |
| Last Modified: | 21 Nov 2022 08:36 |
| URI: | http://irep.iium.edu.my/id/eprint/101062 |
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