Alfanura, Anni'sya and Hayati, Farida and Koestyani, Anindita Dian and Nurkhasanah, Lia and Istikharah, Rochmy and Mohd Nasir, Mohd Hamzah
(2026)
Wound healing activity of Centella asiatica extract via inflammatory gene regulation in Zebrafish (Danio rerio).
Egyptian Pharmaceutical Journal, 25 (4).
pp. 539-546.
ISSN 1687-4315
E-ISSN 2090-9853
Abstract
Background
Centella asiatica (L.) Urb has been recognized for its potential to accelerate wound healing and support tissue repair and regeneration. This effect may involve the regulation of pro-inflammatory genes such as Interleukin-1 Beta (IL-1β) and Matrix Metalloproteinase-9 (MMP- 9).
Objective
This study aimed to evaluate the wound healing potential of ethanolic C. asiatica leaf extract by assessing caudal fin regeneration and the expression levels of IL-1β and MMP-9 in zebrafish (Danio rerio).
Materials and methods
Zebrafish were assigned to control, 7.5-ppm extract, and 10-ppm extract groups (n = 20 per group) and observed for 7 days post-caudal fin amputation. The caudal fin regeneration was quantified by means of a stereo microscope, and inflammatory gene expression (IL-1β and MMP-9) was analyzed using qRT-PCR.
Results and conclusion
The average percentage of caudal fin regeneration was 40.51% in the normal group, 46.32% in the 7.5-ppm extract group, and 47.59% in the 10-ppm extract group. A significant reduction in gene expressions was observed in the 10-ppm group, with –1.097x in IL-1β expression and –0.276x in MMP-9 expression compared to the control.
These findings suggest that C. asiatica extract enhances wound healing in zebrafish by downregulating pro-inflammatory genes
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