Abdul Rahman, Siti Nurul Fazlin and Hamzah, Hairul Aini and Mustafa Mahmoud, Mohammed Imad Al-Deen (2022) Dynamic of mixed genotype HCV infection in plasma and peripheral blood mononuclear cells of chronic hepatitis C hemodialysis patients. In: MSMPS 2022, 10 August 2022, Online. (Unpublished)
|
PDF
Download (1MB) | Preview |
Abstract
Hepatitis C infection is common among dialysis patients. The virological diagnosis is the detection of viral RNA in plasma. It is however reported that HCV can be found in extra-hepatic sites like peripheral blood mononuclear cells (PBMC). Mixed genotype infection may be detected on the same individual which can directly affects the outcome of patients and disease severity. The aim is to investigate the presence of mixed genotype HCV infection in dialysis setting after dual detection of viral RNA in both plasma and PBMC samples. 10 blood samples of dialysis patients with chronic hepatitis C were processed using Ficoll gradient centrifugation. HCV RNA was extracted and subjected to RT-PCR assay using primers targeting The HCV 5'UTR and NS5B regions. DNA sequencing assay was used to obtain their nucleotide sequences. Biedit and Mega 6.4 were used for sequence alignment and constructing phylogenetic tree, respectively. Mixed genotype HCV infection were found in 5 out 10 samples. A combination of HCV genotype 1a and 3a was the main mixed-genotype found in the study. However, there was one sample with combination of genotype 1a, 3a and 6. This may resulted from repeated exposure to HCV. In summary, dual detection on both plasma and PBMC samples demonstrated the presence of mixed-genotype HCV infection in dialysis setting.
| Item Type: | Conference or Workshop Item (Slide Presentation) |
|---|---|
| Subjects: | Q Science > QR Microbiology R Medicine > R Medicine (General) |
| Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): | Kulliyyah of Medicine > Department of Basic Medical |
| Depositing User: | Dr Hairul Aini Hamzah |
| Date Deposited: | 30 Aug 2022 15:08 |
| Last Modified: | 30 Aug 2022 15:08 |
| URI: | http://irep.iium.edu.my/id/eprint/99615 |
Actions (login required)
 |
View Item |
Download Statistics
Download Statistics