Genome size determination of cucumber (cucumis sativus), honeydew (cucumis melo inodorus) and rock Melon (cucumis melo cantalupensis) via flow cytometry

Yumni, Raden Muhamad Imaduddin and Karim, Mohd Fauzihan and Midin, Mohd Razik (2021) Genome size determination of cucumber (cucumis sativus), honeydew (cucumis melo inodorus) and rock Melon (cucumis melo cantalupensis) via flow cytometry. Science Heritage Journal, 5 (1). pp. 14-16. ISSN 2521-0858 E-ISSN 2521-0866

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Abstract

The family of Cucurbitaceae consists of species with economical and nutritional value. Morphologically, there are only few differences between Cucumis species. The interspecific and intraspecific variation in the genome size of the Cucumis species are not discovered yet. Due to this, this study aims to determine the genome size of C. sativus, C. melo inodorus and C. melo cantalupensis using flow cytometry (FCM) method. Nuclei suspension of selected Cucumis species were extracted using LBO1 lysis buffer by manual chopping technique and stained by propidium iodide priot to FCM analysis. Genome size of C. sativus, C. melo inodorus (Honeydew) and C. melo cantalupensis (Rockmelon) were determined by using Glycine max (Soybean) as an external reference standard (2C = 2.5 pg). This study found that the genome size of C. sativus, C. melo inodorus and C. melo cantalupensis estimated to be 2.83 pg, 3.00 pg and 3.47 pg respectively. The genome size data obtained from this study can be used in future genome studies as well as species characterization.

Item Type:	Article (Journal)
Uncontrolled Keywords:	Cucumis species, flow cytometry & genome size
Subjects:	Q Science > QK Botany
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science Kulliyyah of Science > Department of Plant Science
Depositing User:	Dr Mohd Razik Midin
Date Deposited:	10 Aug 2022 15:52
Last Modified:	10 Aug 2022 15:57
URI:	http://irep.iium.edu.my/id/eprint/99342

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