Lymphangiogenesis markers in metastatic lymph nodes of oral squamous cell carcinoma

Hussaini, Haizal and Ibrahim, Nurul Ruziantee and Seo, Benedict and Zain, Rosnah and Rich, Alison (2019) Lymphangiogenesis markers in metastatic lymph nodes of oral squamous cell carcinoma. Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology, 128 (1). e70.

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Abstract

Regional lymph node metastasis is a crucial negative prog- nostic factor in oral squamous cell carcinoma (OSCC). Whilst angiogenic and lymphangiogenic factors have been extensively investigated in primary OSCC, their expression in metastatic lymph nodes remains uncertain. Objectives: To investigate the expression of markers associated with lymphangiogenesis [vascular endothelial growth factor (VEGF)-C, D, VEGF receptor 3 (VEGFR3) and prospero homeobox 1 protein (PROX1)] in cervical lymph nodes from OSCC patients with and without metastatic deposits. Formalin- fixed paraffin-embedded (FFPE) blocks were accessioned from the Oral Cancer Research Coordinating Centre (OCRCC), Uni- versity of Malaya, Malaysia. Samples were divided into two groups; Group A comprised cervical lymph nodes with histologi- cally confirmed metastatic deposits from primary OSCC (n=17) and Group B, cervical lymph nodes from patients with primary OSCC without metastatic deposits, (n=17). Immunohistochemis- try (IHC) was undertaken with antibodies against VEGFC, VEGF-D, VEGFR3 and PROX1. Quantitative analysis using ImageJ was used to delineate the extent of positivity (proportion and intensity) and lymphatic vessel density (LVD). Three sam- ples from each group were subsequently selected for gene expression analysis of the lymphangiogenic markers (VEGFC, VEGFD, VEGFR3 and PROX) using qPCR. Findings: IHC showed significantly greater VEGFC expression in Group A compared with Group B (p=0.0002). Sig- nificant positive correlation was found between VEGFC and TNM stage (p=0.004). No statistically significant differences were observed in the protein and gene expression level of the other tested markers. Conclusions: This is the first study demonstrating sig- nificant overexpression of VEGFC in positive lymph nodes and suggests that VEGFC is an important growth factor involved in OSCC lymph node metastasis.

Item Type:	Article (Meeting Abstract)
Uncontrolled Keywords:	oral squamous cell carcinoma, metastasis
Subjects:	R Medicine > RK Dentistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	UNSPECIFIED
Depositing User:	Dr Nurul Ruziantee Ibrahim
Date Deposited:	28 Jun 2022 16:33
Last Modified:	28 Jun 2022 16:33
URI:	http://irep.iium.edu.my/id/eprint/98506

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