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Rapid Identification of SARS-CoV-2 Lineages from Two Major Clusters in Pahang, Malaysia Using MinION Sequencer

Zainulabid, Ummu Afeera and Wan Ramli, Wan Nurliyana and Aslam, Ayesha and Hussain, Mushtaq and Haroon, Raihanah and Ahmad, Hajar Fauzan (2022) Rapid Identification of SARS-CoV-2 Lineages from Two Major Clusters in Pahang, Malaysia Using MinION Sequencer. In: International Conference on Biomedical Engineering, 21-22 June 2022, Virtual. (Unpublished)

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Background: Rapid identification of variants is needed to take prompt action for public health measures. It was proven that a combination of public health knowledge and Oxford Nanopore Technologies (ONT) could assist in understanding genomic epidemiology. Objective: This study explores the utility of employing the Oxford Nanopore Sequencing in understanding the genomic epidemiology in 2 large clusters in Pahang, Malaysia. Methods: The total RNAs from clinical specimens of combined oropharyngeal and nasopharyngeal swabs representing two major clusters in Pahang, Malaysia, were extracted and proceeded for long-read sequencing. Results: We report 2 variants of concern from this investigation – Beta and Delta variants. We observed that the Taman Tanah Putih Baru and Pasar Kemunting clusters were caused by B.1.351 (ß) and B.1.617 (∆) variants, respectively. Conclusion: Long-read sequencing devices from Oxford Nanopore Technologies (ONT) provide a viable alternative with several benefits. ONT devices are small, inexpensive, and need little laboratory infrastructure or technical expertise to prepare samples. They can also be utilised to perform quick sequencing analysis with flexibility and aid in understanding genomic epidemiology.

Item Type: Conference or Workshop Item (Slide Presentation)
Uncontrolled Keywords: MinION Sequencer, SARS-CoV-2, Covid-19, Beta variant, Delta variant
Subjects: R Medicine > RC Internal medicine > RC111 Infectious and Parasitic Diseases
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Medicine
Kulliyyah of Medicine > Department of Internal Medicine
Depositing User: Asst. Dr. Ummu Afeera Zainulabid
Date Deposited: 21 Jun 2022 15:08
Last Modified: 24 Jun 2022 15:05
URI: http://irep.iium.edu.my/id/eprint/98421

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