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Hepatoprotective effects of a novel trihoney against nonalcoholic fatty liver disease: a comparative study with atorvastatin

Hamad Alfarisi, Hamad Abdulsalam and Ibrahim, Muhammad and Hamad Mohamed, Zenab and Azahari, Nuraniza and Hamdan, Asmah Hanim and Che Mohamad, Che Anuar (2020) Hepatoprotective effects of a novel trihoney against nonalcoholic fatty liver disease: a comparative study with atorvastatin. The Scientific World Journal. pp. 1-14. ISSN 2356-6140 E-ISSN 1537-744X

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Nonalcoholic fatty liver disease (NAFLD) is the most prevalent chronic liver disorder worldwide with no curative therapy. The aim of this study was to investigate the hepatoprotective effects of a novel Trihoney against biochemical and histological manifestations of NAFLD in hypercholesterolemic rabbits. Methodology. Forty-eight male New Zealand white (NZW) rabbits were grouped into normal diet (C), normal diet with 0.6 g/kg/day of Trihoney (C + H), 1% cholesterol diet (HCD), 1% cholesterol diet with 0.3 g/kg/day of Trihoney (HCD + H1), 1% cholesterol diet with 0.6 g/kg/day of Trihoney (HCD + H2), and 1% cholesterol diet with 2 mg/kg/day of atorvastatin (HCD + At.). Animals were sacrificed after 12 weeks of treatment. Serum lipids and liver function test (LFT) were measured prior to and at the endpoint of the experiment for total cholesterol (TC), low-density lipoprotein (LDL-c), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), and total bilirubin (T. Bil.). Liver was processed for histopathology study. Liver homogenate was analysed for oxidative stress parameters: superoxide dismutase (SOD), glutathione peroxidase (GPx), and malondialdehyde (MDA). Results. Lipid analysis approved the induction of hypercholesterolemia. A significant elevation (p < 0.01) of serum AST and ALT levels showed by the HCD group was compared to C and C + H groups. Trihoney exhibited a significant reduction (p < 0.001) of AST and ALT compared to the HCD group. Likewise, AST and ALT reduced significantly in the HCD + At. group (p < 0.001). Trihoney supplementation induced significant (p < 0.05) enhancement of SOD and GPx activities. Atorvastatin treatment was associated with significant (p < 0.05) reduction of SOD and GPx activities in the liver. Trihoney and atorvastatin showed marked (p < 0.001) reduction of hepatic lipid peroxidation. Trihoney showed histological protection against progression of NAFLD to nonalcoholic steatohepatitis (NASH). Atorvastatin exhibited no beneficial impact on hepatic architecture. Conclusion. Trihoney was able to maintain normal liver function and showed hepatoprotection against progression of NAFLD to NASH probably through hypocholesterolaemic and antioxidant functions.

Item Type: Article (Journal)
Subjects: R Medicine > RC Internal medicine > RC803 Specialties of Internal Medicine-Diseases of The Digestive System. Gastroenterology
R Medicine > RM Therapeutics. Pharmacology > RM216 Diet Therapy. Clinical Nutrition
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Allied Health Sciences
Kulliyyah of Allied Health Sciences > Department of Nutrition Sciences
Kulliyyah of Dentistry
Kulliyyah of Medicine
Kulliyyah of Medicine > Department of Pathology & Lab Medicine
Kulliyyah of Pharmacy
Kulliyyah of Pharmacy > Department of Basic Medical Sciences
Depositing User: Dr. Hamad Abdulsalam Hamad Alfarisi
Date Deposited: 02 Jun 2022 08:37
Last Modified: 02 Jun 2022 08:37
URI: http://irep.iium.edu.my/id/eprint/98140

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