Molecular interactions of trichoderma β-1,4-glucosidase (ThBglT12) with mycelial cell wall components of phytopathogenic Macrophomina phaseolina

Mohammad Hood, Mohammad Hakim and Tengku Abdul Hamid, Tengku Haziyamin and Abdul Wahab, Roswanira and Huyop, Fahrul Zaman and Kaya, Yilmaz and Abdul Hamid, Azzmer Azzar (2022) Molecular interactions of trichoderma β-1,4-glucosidase (ThBglT12) with mycelial cell wall components of phytopathogenic Macrophomina phaseolina. Journal of Biomolecular Structure and Dynamics. pp. 1-17. ISSN 0739-1102 E-ISSN 1538-0254

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Abstract

Efficacy of a β-1,4-glucosidase from Trichoderma harzianum T12 (ThBglT12) in disrupting the cell wall of the phytopathogenic fungus M. phaseolina (Macrophomina phaseolina) was studied, as the underlying molecular mechanisms of cell wall recognition remains elusive. In this study, the binding location identified by a consensus of residues predicted by COACH tool, blind docking, and multiple sequence alignment revealed that molecular recognition by ThBglT12 occurred through interactions between the α-1,3-glucan, β-1,3-glucan, β-1,3/1,4-glucan, and chitin components of M. phaseolina, with corresponding binding energies of −7.4, −7.6, −7.5 and −7.8 kcal/mol.

Item Type:	Article (Meeting Abstract)
Uncontrolled Keywords:	Chitin, molecular docking, Trichoderma harzianum, Macrophomina phaseolina, β-1,4-glucosidase, β-1,3/1,4-glucan
Subjects:	S Agriculture > S Agriculture (General) > S494.5.S86 Sustainable agriculture
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Biotechnology
Depositing User:	Dr Tengku Haziyamin Tengku Abd Hamid
Date Deposited:	13 May 2022 15:43
Last Modified:	13 May 2022 15:43
URI:	http://irep.iium.edu.my/id/eprint/97902

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