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Bacterial metabolomics: sample preparation methods

Khairunnisa, Mohd Kamal and Mahamad Maifiah, Mohd Hafidz and Abdul Rahim, Nusaibah and Hashim, Yumi Zuhanis Has-Yun and Abdullah Sani, Muhamad Shirwan and Azizan, Kamalrul Azlan (2022) Bacterial metabolomics: sample preparation methods. Biochemistry Research International, 2022 (9186536). pp. 1-14. ISSN 2090-2247 E-ISSN 2090-2255

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Metabolomics is a comprehensive analysis of metabolites existing in biological systems. As one of the important “omics” tools, the approach has been widely employed in various fields in helping to better understand the complex cellular metabolic states and changes. Bacterial metabolomics has gained a significant interest as bacteria serve to provide a better subject or model at systems level. ,e approach in metabolomics is categorized into untargeted and targeted which serves different paradigms of interest. Nevertheless, the bottleneck in metabolomics has been the sample or metabolite preparation method. A custom-made method and design for a particular species or strain of bacteria might be necessary as most studies generally refer to other bacteria or even yeast and fungi that may lead to unreliable analysis. ,e paramount aspect of metabolomics design comprises sample harvesting, quenching, and metabolite extraction procedures. Depending on the type of samples and research objective, each step must be at optimal conditions which are significantly important in determining the final output. To date, there are no standardized nor single designated protocols that have been established for a specific bacteria strain for untargeted and targeted approaches. In this paper, the existing and current developments of sample preparation methods of bacterial metabolomics used in both approaches are reviewed. ,e review also highlights previous literature of optimized conditions used to propose the most ideal methods for metabolite preparation, particularly for bacterial cells. Advantages and limitations of methods are discussed for future improvement of bacterial metabolomics.

Item Type: Article (Review)
Subjects: Q Science > QD Chemistry
Q Science > QR Microbiology
T Technology > TP Chemical technology
T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): International Institute for Halal Research and Training (INHART)
Depositing User: Dr Mohd Hafidz Mahamad Maifiah
Date Deposited: 18 Apr 2022 08:47
Last Modified: 18 Apr 2022 08:47
URI: http://irep.iium.edu.my/id/eprint/97665

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