Ascorbic acid-loaded poly(lactic-co-glycolic acid) nanoparticles incorporated into a polyacrylic acid gel as a promising tool for site-specific oral cancer therapy

Mohammad Hamdi, Nurul Ain and Harun Ismail, Ahmad Fahmi and Haris @ Harith, Muhammad Salahuddin and Lestari, Widya (2023) Ascorbic acid-loaded poly(lactic-co-glycolic acid) nanoparticles incorporated into a polyacrylic acid gel as a promising tool for site-specific oral cancer therapy. Thai Journal of Pharmaceutical Sciences, 46 (6). pp. 696-710. ISSN 0125-4685 E-ISSN 1905-4637

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Abstract

Background: Chemotherapy is commonly used in oral cancer therapy, especially as the disease advances. However, it is associated with terrible adverse effects and the occurrence of chemoresistance which causes treatment failure. Thus, discovering a new potential anticancer agent and developing a safe, effective and non-invasive drug delivery are necessary. Objective: The objective of the current study is to develop ascorbic acid-loaded poly(lactic-co-glycolic) acid (AA-PLGA) nanoparticles incorporated into polyacrylic acid gel intended to treat oral cancer. Materials and methods: Double emulsion solvent evaporation method was used to fabricate AA-PLGA nanoparticles. Optimisation was carried out in the primary emulsion based on multilevel factorial design by testing at varying surfactant types and concentrations. The optimised nanoparticles formulation was further incorporated into different concentrations of polyacrylic acid gel, and compared with a mucoadhesive polyacrylic acid-based commercial product (Kin Care) as a reference. The optimised AA-PLGA nanoparticles were subjected to cytotoxic assay against the SCC-25 cell line. Results: For the optimised formulation, we observed particle size of 252 ± 2.98 nm, polydispersity index (PDI) of 0.151 ± 0.02, zeta potential of -20.93 ± 0.87 mV, and encapsulation efficiency of 69.73 ± 1.07%. Polyacrylic acid polymer with a strength of 1% was chosen as the optimum gelling agent for AA-PLGA nanoparticles-in-gel formulation. Cytotoxicity study of the optimised nanoparticle demonstrated significant (p-value < 0.05) reduction of cancer cell viability in a dose-dependent manner with a half-maximal inhibitory concentration value of 2.42 mg/mL. Conclusion: The results of the present study support the feasibility of AA-PLGA nanoparticles-in-gel formulation for oral cancer therapy.

Item Type:	Article (Journal)
Uncontrolled Keywords:	Ascorbic acid; anti-cancer; PLGA; mucoadhesive gel; oral cancer; nanoparticles
Subjects:	R Medicine > RS Pharmacy and materia medica
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences Kulliyyah of Allied Health Sciences > Department of Physical Rehabilitation Sciences Kulliyyah of Pharmacy Kulliyyah of Pharmacy > Department of Pharmaceutical Technology
Depositing User:	Dr. Muhammad Salahuddin Haris
Date Deposited:	05 Apr 2022 12:08
Last Modified:	04 Sep 2023 15:25
URI:	http://irep.iium.edu.my/id/eprint/97489

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