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Analysis of phenolic profile, total phenolic content and antioxidant activity in Anacardium occidentale leaves

Sassi, Assia and Haron, Normah and Khattak, Muhammad Muzaffar Ali Khan and Mat Jusoh, Hanapi (2022) Analysis of phenolic profile, total phenolic content and antioxidant activity in Anacardium occidentale leaves. Food Research, 6 (1). pp. 20-26. E-ISSN 2550-2166

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Anacardium occidentale young leaves are consumed traditionally as part of a Southeast Asian diet. The regular consumption is believed to have beneficial effects on health in general and potentially against type 2 Diabetes mellitus due to its high content of polyphenols. This study was aimed to investigate the polyphenol content of the plant using two methanol extracts; Free Phenolic Extract (FPE) and Bound Phenolic Extract (BPE) as well as highlight the presence of six phenolic acids and flavonoids namely; gallic acid, sinapinic acid, caffeic acid, ferulic acid, quercetin and kaempferol using High performance liquid chromatography-photodiode array (HPLC-UV-Vis). The results for polyphenols and flavonoids content showed high amounts of total polyphenols in BPE with 8.5±0.57 mg GAE/g as well as high amounts of total flavonoids in both extracts FPE and BPE with 0.58±0.06 and 0.86±0.05 mg QE/g respectively (p<0.05). The presence of these polyphenols was further confirmed by measuring the antioxidant activity through the scavenging of the free radical DPPH which showed an IC50 value for FPE (5.17±0.64 µg/ mL, BPE (4.96±0.12 µg/mL) compared to the positive control ascorbic acid (4.91±0.43 µg/mL). The high-performance liquid chromatography-photodiode array confirmed the presence of all four targeted phenolic acids with the highest amount showing in gallic acid and sinapinic acid in BPE with 148.12±6.44 µg gallic acid/g dry weight and 47.02±1.94 µg sinapinic acid/g dry weight respectively. As for flavonoids, quercetin was present in both extracts with 20.38±1.22 µg/g dry weight in BPE and 5.21±0.1 µg/g dry weight in FPE while Kaempferol was not detectable in either extract. These findings confirmed the importance of A. occidentale as a rich source of polyphenols which can be further investigated to determine its effects in-vitro and in-vivo on non-communicable diseases such as type 2 diabetes.

Item Type: Article (Journal)
Uncontrolled Keywords: Anacardium occidentale, Phenolics acids, Flavonoids, HPLC, Antioxidants
Subjects: Q Science > Q Science (General)
Q Science > QD Chemistry
R Medicine > RV Botanic, Thomsonian, and eclectic medicine
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Allied Health Sciences
Kulliyyah of Allied Health Sciences > Department of Nutrition Sciences
Depositing User: Dr. Normah Haron
Date Deposited: 12 Jan 2022 09:09
Last Modified: 01 Apr 2022 08:25
URI: http://irep.iium.edu.my/id/eprint/96214

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