In vitro angiotensin-converting enzyme (ACE) inhibition activity of Syzygium polyanthum leaves and its inhibition mechanism

Ismail, Azlini and Tuan Anuar, Tuan Ashraf Faiz and Mohamed Suffian, Izzat Fahimuddin and Abdul Hamid, Azzmer Azzar and Omar, Muhammad Nor (2021) In vitro angiotensin-converting enzyme (ACE) inhibition activity of Syzygium polyanthum leaves and its inhibition mechanism. In: Frontiers in Pharmacology. Frontiers. ISBN 978-2-88971-008-9

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Abstract

Syzygium polyanthum is an ethnomedicinal plant with significant antihypertensive effect; however, the mechanism of action is not yet well-described. This study aims to investigate one of its possible antihypertensive mechanisms, the angiotensin-converting enzyme (ACE) inhibition and its inhibition mechanism. S. polyanthum leaves were macerated using water, methanol, ethyl acetate, and hexane to produce the aqueous (ASP), methanolic (MSP), ethyl acetate (EASP) and hexane (HSP) extracts. Each extract (100 μg/ml) was screened for in vitro ACE inhibition activity according to Cushman and Cheung assay method. The standard drug, captopril (2.06 ng/ml) was used as a positive control. The most active extract was further tested at 1 to 1000 μg/ml to determine its potency. A similar assay was then conducted in the presence of zinc chloride or bovine serum albumin (BSA) to determine ASP’s ACE inhibition mechanism. ASP was found to exhibit the highest inhibition activity (69.43 ± 0.60 %), followed by HSP (45.40 ± 0.15 %), MSP (41.63 ± 0.15 %), and EASP (9.62 ± 1.60 %). The concentration of ASP that caused 50 % ACE inhibition activity (IC50) was 41 μg/ml. ASP’s ACE inhibition activity was significantly reduced with the presence of BSA but not significantly affected by the presence of zinc chloride. In conclusion, S. polyanthum leaves possessed a significant ACE inhibition activity which was majorly present in its aqueous extract. It is postulated that ASP’s ACE inhibition activity was due to its ability to conjugate with protein, and it was non-dependent on the zinc at the ACE active site.

Item Type:	Book Chapter
Uncontrolled Keywords:	Angiotensin-converting enzyme, Antihypertensive, Hypertension, Syzygium polyanthum
Subjects:	Q Science > Q Science (General) Q Science > QA Mathematics Q Science > QD Chemistry R Medicine > RM Therapeutics. Pharmacology R Medicine > RM Therapeutics. Pharmacology > RM300 Drugs and their action
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Dentistry Kulliyyah of Dentistry > Department of Fundamental Dental and Medical Sciences Kulliyyah of Pharmacy Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry Kulliyyah of Science Kulliyyah of Science > Department of Biotechnology Kulliyyah of Science > Department of Chemistry
Depositing User:	DR. AZLINI ISMAIL
Date Deposited:	29 Dec 2021 20:37
Last Modified:	29 Dec 2021 20:37
URI:	http://irep.iium.edu.my/id/eprint/95580

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