Chronic exposure of low-dose organic arsenic induced oxidative stress injury on the rats kidney

Wan Salleh, Wan Muhamad Salahudin and A.Talib, Norlelawati and Abdullah, Nor Zamzila and Saharudin, Shahida and Aung, Sanda and Buyong, Zunariah (2021) Chronic exposure of low-dose organic arsenic induced oxidative stress injury on the rats kidney. In: Medical Research Symposium 2021, 14 December 2021, Kuantan, Pahang. (Unpublished)

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Abstract

Introduction: Monosodium methylarsonate (MSMA) is an organic arsenical herbicide widely used in agriculture. Humans are exposed to arsenic through contaminated drinking water and anthropogenic activities. Exposure to acute and high dose of inorganic arsenic induced oxidative stress injury in the tissue. However, studies focusing on effects of chronic low dose organic arsenic exposure on oxidative genes of kidneys are limited. Therefore, the objective of this study is to determine the effects of chronic low dose organic arsenic exposure on rats’ kidneys oxidative genes at 6-month duration. Materials and Methods: Twelve male Sprague-Dawley rats, aged 2-3 months old, with average weight of 200 to 250g divided into two groups (n=6); control and treatment respectively. The control group was given daily oral gavage of distilled water and the treatment group was given daily oral gavage of MSMA at 63mg/kg body weight for 6-months duration. Kidneys were harvested for histology and gene expression analysis. Results: Histologically, on H&E & PAS staining, the control group showed normal appearance of the glomerular and tubular structures. The treatment groups showed microscopic changes of the glomerular and tubular structures such as glomerular atrophy with widening of Bowman’s space, dilated tubular lumen, loss of brush border and presence of tubular cast. Gene’s analysis showed upregulation of Glutathione reductase (GSR) and downregulation of Catalase and nitrite oxide synthase 1 (NOS1). Conclusion: Chronic exposure to low dose organic arsenic induced kidney injury through oxidative stress mechanism.

Item Type:	Conference or Workshop Item (Poster)
Additional Information:	6299/95395
Uncontrolled Keywords:	organic arsenic, oxidative stress, kidney injury
Subjects:	R Medicine > RA Public aspects of medicine R Medicine > RA Public aspects of medicine > RA1190 Toxicology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Medicine > Department of Basic Medical Kulliyyah of Pharmacy > Department of Basic Medical Sciences Kulliyyah of Medicine Kulliyyah of Medicine > Department of Pathology & Lab Medicine Kulliyyah of Pharmacy
Depositing User:	dr wan muhamad salahudin wan salleh
Date Deposited:	29 Dec 2021 16:23
Last Modified:	29 Dec 2021 16:23
URI:	http://irep.iium.edu.my/id/eprint/95395

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