The effects of Tualang honey with or without diet modifications on sperm profile in high cholesterol diet induction animal model

Hasan, Sakiinah and Abdul Rahim, Roslina and Alias, Mohd Afzal and Muhammad, Siti Aeshah @ Naznin and Abdullah, Nor Zamzila and Abdul Razak, Redzuan Nul Hakim (2021) The effects of Tualang honey with or without diet modifications on sperm profile in high cholesterol diet induction animal model. In: IIUM Research Day 2021: An International Online Event for Life Sciences, 24th November 2021, Kuantan, Pahang. (Unpublished)

	PDF (Acceptance letter) - Supplemental Material Restricted to Repository staff only Download (129kB) | Request a copy
	PDF (Poster presentation) - Presentation Restricted to Registered users only Download (244kB) | Request a copy
	PDF (Abstract) - Supplemental Material Restricted to Registered users only Download (80kB) | Request a copy

Abstract

Introduction: Hypercholesterolaemia is a recognised factor associated with male infertility. However, currently, there is limited therapy available. Tualang honey (TH) is a type of Malaysian polyfloral wild honey produced by the rock bee (Apis dorsata) that has been proven to exert both anti-inflammatory and anti-oxidative effects. This study aimed to determine the effects of TH with or without diet modifications on the sperm profile high cholesterol diet (HCD) administered rats. Methods: Thirty Sprague Dawley rats weighing 200 - 250 gm were randomly divided into the standard diet (control) group (n=6), mixed cholesterol diet (MCD) group and high cholesterol diet (HCD) group (n=12 each). The six rats in the control group were fed with standard commercial rat pellet while the 12 rats in the MCD and HCD group were fed with a 12% cholesterol diet for 16 weeks. At the end of the 16 weeks, the rats in the MCD group were divided into two subgroups (M1 and M2) and continued on standard diet in addition to administration of distilled water and 1.2 g of honey per kg body weight daily respectively for 4 weeks. The rats in the HCD group were divided into two subgroups (H1 and H2) and continued on the 12% high cholesterol diet in addition to administration of distilled water and 1.2 g of honey per kg body weight daily respectively for 4 weeks. The rats in the control group were continued with commercial rat pellets without honey supplementation. At the end of the 4 weeks, all rats were sacrificed and the cauda epididymis was collected for sperm analysis. Results: The sperm parameters (sperm concentration, the percentage of total sperm motility, progressive motility, normal sperm morphology, and viability) of rats in group M1 and H1 were significantly reduced compared to the control. In contrast, all TH supplemented groups demonstrated significant improvement in the sperm parameters. However, there were significant increase in the sperm concentration and normal sperm morphology in group M2 compared to H2. Conclusion: In conclusion, the TH supplementation with diet modifications improved the sperm analysis results in the animal model.

Item Type:	Conference or Workshop Item (Poster)
Additional Information:	5456/95308
Uncontrolled Keywords:	High cholesterol diet, Tualang honey, Follicular-stimulating hormone, sperm profile
Subjects:	Q Science > QP Physiology R Medicine > R Medicine (General) R Medicine > RB Pathology R Medicine > RC Internal medicine R Medicine > RM Therapeutics. Pharmacology R Medicine > RM Therapeutics. Pharmacology > RM214 Diet therapy. Diet and dietectics in disease
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Medicine Kulliyyah of Medicine > Department of Basic Medical Kulliyyah of Nursing Kulliyyah of Nursing > Department of Basic Medical Sciences for Nursing
Depositing User:	Dr ROSLINA ABDUL RAHIM
Date Deposited:	28 Dec 2021 10:29
Last Modified:	07 Apr 2022 09:49
URI:	http://irep.iium.edu.my/id/eprint/95308

Actions (login required)

View Item