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The effect of Momordica Carantia (Bitter Mellon) Extracts on human Adenocarcinoma Cells Proliferation and Apoptosis

Ahmad Sujangi, Nurhikmah and Jamil, Elyna Fatini and Abdul Ghani, Radiah (2021) The effect of Momordica Carantia (Bitter Mellon) Extracts on human Adenocarcinoma Cells Proliferation and Apoptosis. In: 9th International Symposium on Applied Engineering and Sciences 2021, Kuala Lumpur. (Unpublished)

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Abstract: Chemoprevention is a crucial process in overcoming the cancer burden throughout the world. By changing dietary choices, people who are at high risk of developing cancer may slow down the carcinogenesis or preventing it altogether. Some food contains high level of polyamine which is bad due to its ability for promoting tumor growth. Hence, this research aimed to determine the polyamine content of selected local plant, Momordica charantia by using High Performance Liquid Chromatographic (HPLC) analysis as well as conducting cytotoxicity study and growth inhibition study of the plant extracts against the human lung adenocarcinoma epithelial cell lines, A549. The type of cell death and its pathway also were elucidated. The polyamine content of M. charantia was found to be high (more than 200 nmol/g/mol). It is found to induce cytotoxicity against A549 cells after only 24 hours of exposure which lead to the establishment of the IC50 at 10.0 mg/ml. Growth curve analysis demonstrated that M. charantia had successfully reduced the cancer cell progression. On the other hand, there was an increase of intracellular polyamines in the treated cells compared to untreated cells. Apoptosis was induced Based on these preliminary findings, it can be concluded that M. charantia and G. procumbens are not an excellent plants to be included in the development of chemoprevention strategy as the ideal chemopreventive agents are those that contain low polyamine level, able to reduce the cancer cell progression and can inhibit the biosynthesis of polyamine in cancer cells to avoid recurrence of cancer. However, further investigation on the effect of these plants on polyamine metabolic pathway and cell death pathway shall be elucidated.

Item Type: Conference or Workshop Item (Plenary Papers)
Additional Information: 4458/94717
Uncontrolled Keywords: chemoprevention, polyamines, cell death, A549
Subjects: R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011)
Depositing User: Dr. Radiah Abdul Ghani
Date Deposited: 29 Dec 2021 18:01
Last Modified: 17 Feb 2022 16:11
URI: http://irep.iium.edu.my/id/eprint/94717

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