Bitter gourd honey ameliorates hepatic and renal diabetic complications on type 2 diabetes rat models by antioxidant, anti-Inflammatory, and anti-apoptotic mechanisms

Arigela, Chandra Sekhar and Nelli, Giribabu and Siew Hua, Gan and Kuttulebbai Nainamohamed Salam, Sirajudeen and Krishnan, Kumarathevan and Abdul Rahman, Nurhanan and Pasupuleti, Visweswara Rao (2021) Bitter gourd honey ameliorates hepatic and renal diabetic complications on type 2 diabetes rat models by antioxidant, anti-Inflammatory, and anti-apoptotic mechanisms. Foods, 10 (11). ISSN 2304-8158

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Abstract

Abstract: Honey has several pharmacological effects, including anti-diabetic activity. However, the effectiveness of bitter gourd honey (BGH) in the treatment of diabetes mellitus (DM) is unknown. The aim of this study was to determine the antioxidant, anti-inflammatory, and anti-apoptotic properties of BGH on the kidney and liver of a streptozotocin-induced diabetes rat model. Methods: A single dose (nicotinamide 110 mg/kg, streptozotocin (STZ) 55 mg/kg, intraperitoneal (i.p.)) was used to induce DM in male rats. For 28 days, normal or diabetic rats were administered 1 g/kg/day and 2 g/kg/day of BGH orally. After the treatment, blood, liver, and kidney samples were collected and analysed for biochemical, histological, and molecular parameters. In addition, liquid chromatography–mass spectrometry (LC-MS) was used to identify the major bioactive components in BGH. Results: The administration of BGH to diabetic rats resulted in significant reductions in alanine transaminase (ALT),aspartate aminotransferase (AST), creatinine, and urea levels. Diabetic rats treated with BGH showed lesser pathophysiological alterations in the liver and kidney as compared to non-treated control rats. BGH-treated diabetic rats exhibited reduced levels of oxidative stress (MDA levels), inflammatory (MYD88, NFKB, p-NFKB, IKK�), and apoptotic (caspase-3) markers, as well as higher levels of antioxidant enzymes (SOD, CAT, and GPx) in the liver and kidney. BGH contains many bioactive compounds that may have antioxidative stress, anti-inflammatory, and anti-apoptotic effects. Conclusion: BGH protected the liver and kidney in diabetic rats by reducing oxidative stress, inflammation, and apoptosis-induced damage. As a result, BGH can be used as a potential therapy to ameliorate diabetic complications.

Item Type:	Article (Journal)
Uncontrolled Keywords:	Keywords: diabetes mellitus; oxidative stress; antioxidant enzymes; inflammation
Subjects:	R Medicine > R Medicine (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Medicine > Department of Basic Medical Kulliyyah of Medicine
Depositing User:	Prof.Dr. SIRAJUDEEN KUTTULEBBAI NAINAMOHAMED SALAM
Date Deposited:	03 Dec 2021 13:08
Last Modified:	23 Dec 2021 14:55
URI:	http://irep.iium.edu.my/id/eprint/94344

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