Characterization of cervus timorensis velvet antler and its effect on biofilm formation of candida specieson biofilm formation of candida species

Mohd Badri, Puteri Elysa Alia and Rismayuddin, Nurul Alia Risma and Mohd Kenali, Norzaiti and Darnis, Deny Susanti and Arzmi, Mohd Hafiz (2021) Characterization of cervus timorensis velvet antler and its effect on biofilm formation of candida specieson biofilm formation of candida species. In: The 4th International Conference on Oral Microbiology and Oral Immunology (ICOMOI), 6-8 September 2021, Kulliyyah of Dentistry, International islamic University Malaysia, Kuantan Campus. (Unpublished)

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Abstract

Objectives: To characterize deer velvet antler (DVA) extract and to determine its effect on biofilm formation of Candida species. Design: Liquid-Chromatography-Quadrupole-Time of Flight (LC-Q-TOF-MS) was conducted to characterize the secondary metabolites of DVA extract. Candida albicans ATCC MYA-4901 (ALT5), AIDS isolate (ALC2), oral cancer isolate (ALC3), Candida dublieniesis ATCC MYA-2975 (CD), Candida glabrata ATCC 90030 (CG), Candida krusei 14243 (CK), Candida lusitaniae ATCC 34449 (CL), Candida parapsilosis ATCC 22019 (CP) and Candida tropicalis ATCC 13803 (CT) were used in the biofilm study. To determine the monospecies of Candida biofilm, 200 μL of RPMI-1640 containing 3x104 of Candida cells were pipetted into the same well of a 96-well plate. To determine the polymicrobial biofilm formation by Candida, 200 μL of RPMI-1640 containing 3 x 104 of Candida cells and 40 mg of DVA extract were inoculated in the same well. Wells that only consisted of Candida cells served as the negative control. The biofilms were incubated at 37 °C for 72 hours. Finally, a crystal violet (CV) assay was used to quantify biofilm formation and Candida total cell count was enumerated. Results: A total of 46 compounds were detected in the DVA extract. CL exhibited a high percentage of biofilm biomass reduction when treated with DVA extract (66.10), followed by ALC3 (44.12). Besides, Candida exhibited a decrease in total cell count when treated with DVA, except for ALC3 and CK. CG and CP had a similar total cell count when cultured with DVA with 1.17 x 105 cells/mL, while ALT5 had the lowest total cell count when cultured with DVA extract with 0.17 x 105 cells/mL. Conclusion: All Candida species inhibit biofilm formation when cultured with DVA extract except for CG, CK, and CP.

Item Type:	Conference or Workshop Item (Slide Presentation)
Additional Information:	6469/93968
Uncontrolled Keywords:	Deer velvet antler (DVA), Cervus timorensis, Candida species, biofilm
Subjects:	R Medicine > RK Dentistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Dentistry
Depositing User:	AP Ts Dr Mohd Hafiz Arzmi
Date Deposited:	23 Nov 2021 14:31
Last Modified:	23 Nov 2021 14:31
URI:	http://irep.iium.edu.my/id/eprint/93968

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