IIUM Repository

DHA-rich fish oil and Tualang honey reduce chronic stress-induced oxidative damage in the brain of rat model

Asari, Mohd Asnizam and Kuttulebbai Naina Mohamed Salam, Sirajudeen and Mohd Yusof, Nurul Aiman and Mohd Amin, Mohamad Syabil Ikhwan (2022) DHA-rich fish oil and Tualang honey reduce chronic stress-induced oxidative damage in the brain of rat model. Journal of Traditional and Complementary Medicine, 12 (4). pp. 361-366. ISSN 2225-4110

PDF (Full text article) - Published Version
Download (872kB) | Preview
[img] PDF (Scopus evidence) - Supplemental Material
Restricted to Repository staff only

Download (671kB) | Request a copy


Background: Exposure to chronic stress induces oxidative damage which alters the dynamic balance between antioxidant and pro-oxidant activities in the brain. Tualang honey (TH) is a Malaysian wild multifloral honey which has been shown to contain high amount antioxidants. DHA-rich fish oil is a form of omega-3 fatty acids found in fish which also possesses some antioxidant activity. This study aimed to evaluate anti-stress activity of DHA-rich fish oil, TH and their combination on several parameters of oxidative stress in chronic stress rat model. Methods: Fifty male Sprague Dawley rats were divided into (i) control, (ii) stress-exposed, (iii) stressexposed and treated with TH (1 g/kg body weight twice daily), (iv) stress-exposed and treated with DHA-rich fish oil (450 mg/kg body weight twice daily), and (v) stress-exposed and treated with a combination of TH and DHA-rich fish oil. The chronic stress regimen consisted of a combination of restraint stress and a swim stress test for 28 days. Results: DHA-rich fish oil and TH significantly (p < 0.05) supressed stress-induced elevation of serum corticosterone and lipid peroxidation, and caused a significant increase in total antioxidant capacity. For glutathione status, only TH significantly reduced stress-induced elevation of oxidised glutathione (GSSG) and normalised GSH/GSSG ratio. Conclusion: Both DHA-rich fish oil and TH have protective effects against brain oxidative stress but consuming these substances together does not seem to provide an additional benefit compared to consuming them separately.

Item Type: Article (Journal)
Additional Information: 9547/93882
Uncontrolled Keywords: Oxidative stress, Honey, DHA, Omega-3, Restraint stress
Subjects: R Medicine > R Medicine (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Medicine
Kulliyyah of Medicine > Department of Basic Medical
Depositing User: Mrs Norsyafini Ahmad Marzuki
Date Deposited: 25 Nov 2021 12:12
Last Modified: 29 Nov 2022 11:15
URI: http://irep.iium.edu.my/id/eprint/93882

Actions (login required)

View Item View Item


Downloads per month over past year