The protective effect of sodium nitroprusside(SNP)treatment on Arabidopsis thaliana seedlings exposed to toxic level of Pb is not linked to avoidance of Pb uptake

Phang, Ing Chia and Leung, David W. M. and Taylor, H. Harry and Burritt, David J. (2011) The protective effect of sodium nitroprusside(SNP)treatment on Arabidopsis thaliana seedlings exposed to toxic level of Pb is not linked to avoidance of Pb uptake. Ecotoxicology and Environmental Safety, 74 (5). pp. 1310-1315. ISSN 0147-6513

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Abstract

The effects of 100 μM Pb(NO3)2 on 7-day-old Arabidopsis thaliana seedlings grown from seeds pretreated with a nitric oxide donor, sodium nitroprusside (SNP), or a nitric oxide scavenger, 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO) were investigated. Physiological stress induced by Pb (reduced root growth) was less evident in seedlings grown from seeds pretreated with SNP. However, SNP pretreatment of seeds did not affect Pb accumulation in the seedlings. Pb exposure caused oxidative stress by elevating hydrogen peroxide (H2O2) and lipid hydroperoxide contents of the seedlings. SNP pretreatment of seeds counteracted Pb toxicity by reducing the H2O2 and lipid hydroperoxide contents of Pb-exposed seedlings. Additionally, Pb-induced rises in antioxidative enzyme activities were reversed by SNP pretreatment of seeds. It was concluded that the ameliorating effects of SNP pretreatment were associated with the release of nitric oxide because cPTIO reversed these effects of SNP pretreatment of seeds and more importantly SNP pretreatment did not trigger an avoidance mechanism.

Item Type:	Article (Journal)
Additional Information:	6480/938
Uncontrolled Keywords:	Antioxidative enzymes; Heavy metals; Lead; Oxidative stress; Phytoremediation
Subjects:	Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Biotechnology
Depositing User:	Dr. Ing Chia Phang
Date Deposited:	22 Aug 2011 10:48
Last Modified:	21 Jun 2013 11:40
URI:	http://irep.iium.edu.my/id/eprint/938

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