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Disinfection protocol on irreversible hydrocolloids: effect on bacteria count and dimensional accuracy

Ahmad Zawawi, Nur Dini and Muhammad, Nur Farah Athirah and Ahmad, Norfaezah and Nasir, Siti Hajjar and Arzmi, Mohd Hafiz (2021) Disinfection protocol on irreversible hydrocolloids: effect on bacteria count and dimensional accuracy. In: The 10th Dental Students' Scientific Conference, 27 April 2021, Kulliyyah of Dentistry, International islamic University Malaysia, Kuantan Campus. (Unpublished)

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Abstract

Introduction: Disinfection of impression is compulsory to prevent cross-infection to dental personnel. However, prolonged immersion in disinfectants may affect the dimensional accuracy of irreversible hydrocolloid. Different types of disinfectants are available nowadays, including alcohol-based, glutaraldehyde, and chlorine derivatives. Aim and objectives: To investigate the disinfection protocols and their effect on Candida count and dimensional accuracy of irreversible hydrocolloid. Materials and methods: For the antifungal activity part, 60 alginate beads were made and treated with different disinfection protocols (2% Aseptoprint, 1% sodium hypochlorite, 3% MD 520 for two minutes, one hour, six hours or 24 hours) except for control group. The beads were contaminated with Candida a/bicans (ATCC MYA 4901). Colony-forming units (CFUs) were counted using a haemocytometer and analysed using two-way ANOVA. For dimensional accuracy assessment, alginate impressions were taken from a master cast and treated with the same disinfection protocols. Three linear measurements of casts from impressions immersed in different disinfection protocols were compared to the master cast. Statistical analysis was determined using Friedman Test. Results: There was a significant difference in the reduction of CFUs after disinfection with 3% MD 520 and 2% Aseptoprint (P<0.05). The dimensional changes of alginates treated for six and 24 hours were statistically significant. Discussion: 3% MD 520 and 2% Aseptoprint had effective antifungal activity on irreversible hydrocolloids impression. Dimensional changes of alginate were due to imbibition and syneresis, affecting the casts constructed. Longer immersio111 time yielded less fungal count but resulted in increased dimensional changes. Conclusions: 3% MD 520 and 2% Aseptoprint are recommended for irreversible hydrocolloid disinfec­tion, with immersion time not exceeding one hour.

Item Type: Conference or Workshop Item (Poster)
Uncontrolled Keywords: Infection control, irreversible hydrocolloid, antifungal, dimensional accuracy
Subjects: Q Science > QR Microbiology
R Medicine > RK Dentistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Dentistry > Department of Prosthodontics
Depositing User: Dr Mohd Hafiz Arzmi
Date Deposited: 28 Oct 2021 10:00
Last Modified: 28 Oct 2021 10:00
URI: http://irep.iium.edu.my/id/eprint/93153

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