Antioxidants profile of momordica charantia fruit extract analyzed using LC-MS-QTOF-based metabolomics

Perumal, Vikneswari and Khatib, Alfi and Ahmed, Qamar Uddin and Uzir, Bisha Fathamah and Abas, Faridah and Murugesu, Suganya and Saiman, Mohd Zuwairi and Primaharinastiti, Riesta and El-Seedi, Hesham (2021) Antioxidants profile of momordica charantia fruit extract analyzed using LC-MS-QTOF-based metabolomics. Food Chemistry: Molecular Sciences, 2. ISSN 2666-5662

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Abstract

Momordica charantia fruit is claimed to have healthy benefit. Despite this potential claim, the phytochemical study of this fruit is still lacking. Thus, this study aimed to evaluate the antioxidants profile of Momordica charantia (Cucurbitaceae) fruit. The antioxidant activity of the ethanolic extracts of various polarities was evaluated and the metabolites that are responsible for its activity were identified using metabolomics approach. Six different mixture of ethanol in water that are 0%, 20%, 40%, 60%, 80%, and 100% (v/v) was extracted using fruit sample. Liquid chromatography‐mass spectrometry‐quadrupole time of flight and multivariate data analysis was used to identify the metabolites that were either antioxidants or pro‐oxidants. The 80% ethanol extract exhibited the most antioxidant activity when tested in both 2, 2‐diphenyl‐1‐picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) antioxidant assays. This extract showed the most intense LC‐MS signals represented to ascorbic acid, margarolic acid, brevifolincarboxylic acid, quercetin 3‐O‐glycoside, kuguacin H, cucurbitacin E, 3‐malonylmomordicin I, and goyaglycoside G correlating to the anti‐oxidant activity. This study reports for the first time the existence of brevifolincarboxylic acid in this fruit, and the antioxidant activity of 3‐malonylmomordicin I and goyaglycoside G. In addition, the loading plots revealed the unknown compounds possessing the antioxidant activity which are potential to be isolated in the future study.

Item Type:	Article (Journal)
Additional Information:	6915/93042
Uncontrolled Keywords:	Antioxidants, DPPH, FRAP.LC‐MS‐QTOF, Metabolomics, Momordica charantia
Subjects:	Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry Kulliyyah of Pharmacy
Depositing User:	Dr. Alfi Khatib
Date Deposited:	15 Oct 2021 11:02
Last Modified:	15 Oct 2021 11:02
URI:	http://irep.iium.edu.my/id/eprint/93042

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