Purification and Screening of Selected Microbes for Biotransformation of Xanthorrizolfrom the Essential Oil of Curcuma xanthorrhiza

Yahya, Nadia Farhana and Aminudin, Nurul Iman and Zainal Abidin, Zaima Azira and Darnis, Deny Susanti and Bakhtiar, M. Taher (2021) Purification and Screening of Selected Microbes for Biotransformation of Xanthorrizolfrom the Essential Oil of Curcuma xanthorrhiza. In: 33rdInternational Conference of Analytical Sciences (SKAM33, 13 -15 September 2021, Virtual. (Unpublished)

Preview	PDF Download (270kB) | Preview
	PDF Download (16MB)

Abstract

Xanthorrhizol, a bisabolene-type sesquiterpenoid is the most active and abundant component present in the essential oil of Curcuma xanthorrhiza (temulawak) [1]. It was reported to possess various pharmacological activities that includes antimicrobial, anti-inflammatory, antioxidant, antihyperglycemic, antihypertensive, antiplatelet, nephroprotective and hepatoprotective, estrogenic and antiestrogenic properties [2]. To further evaluate its pharmacological potency based on the structure-activity relationship, abundance amount of xanthorrhizol need to be purified and subject to chemical synthesis to yield the xanthorrhizol analogues. Common approaches to synthesize the analogues is through the chemical reactions. Biotransformation utilizing microbes as biocatalysts served as one of the green alternatives replacing chemical synthesis method which able to yield potential xanthorrhizol analogues. In this study, xanthorrhizol will be purified from the crude essential oil utilizing chromatographic and two steps chemical synthesis techniques. The structure and purity of xanthorrhizol were determined through spectroscopic analysis. Selected microbes i.e Streptomyces sp. and Aspergillus sp. will be screened as potential biocatalysts for the biotransformation reaction. Thin layer chromatography (TLC) and Gas Chromatography (GC) were used to monitor the presence of biotransformation product.

Item Type:	Conference or Workshop Item (Poster)
Subjects:	Q Science > QD Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Chemistry Kulliyyah of Science
Depositing User:	Dr Nurul Iman Aminudin
Date Deposited:	21 Sep 2021 14:41
Last Modified:	21 Sep 2021 14:41
URI:	http://irep.iium.edu.my/id/eprint/92385

Actions (login required)

View Item