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Optimization of CNTs production using full factorial design and its advanced application in protein purification

Mubarak, N. M. and Yusof, Faridah and Al-Khatib, Ma An Fahmi Rashid and Ameen, Emad S.M and Khalid, M. and Al-Sa'adi, Abdul Razak Abdul Rahman Asaad and Atieh, Muataz A. and Qudsieh, Isam Y. and Walvekar , Rashmi Gangasa (2010) Optimization of CNTs production using full factorial design and its advanced application in protein purification. International Journal of Nanoscience, 9 (3). pp. 181-192. ISSN 0219-581X (P), 1793-5350 (O)

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Abstract

Carbon nanotubes (CNTs) have been successfully synthesized by using in-house fabricated Double Stage Chemical Vapor Deposition (DS-CVD) technique, using acetylene (C2H2) and hydrogen (H2) as the precursor gases. The purity, morphology and the structure of CNTs were then characterized using Field Emission Scanning Microscope (FESEM), Transmission Electron Microscope (TEM) and Thermogravimetric Analysis (TGA). The effects of the process parameters were examined whereby the experimental design of the investigation was conducted using Design Expert® Version 6.0.8. The statistical analysis reveals that the optimized conditions for the best CNTs yield production at 850°C reaction temperature, 60 min reaction time, with gas flow rates at 40 and 150 ml/min for C2H2 and H2, respectively. The CNTs produced were successfully used as column chromatographic media. Due to its nanosized structured dimension, CNTs' have tremendously large surface area and that lead to highly efficient protein purification. Skim latex protein has been used as the model protein and we aim to recover useful proteins and enzymes from this known wasteful material. During the purification, the process parameters such as pH and ionic strength of the running buffer were optimized to enhance protein purification. Results reveal that CNTs behave efficiently as a hydrophobic interaction chromatographic media.

Item Type: Article (Journal)
Additional Information: 4734/9230
Uncontrolled Keywords: CNTs; DS-CVD; functionalization; skim latex serum; protein purification.
Subjects: T Technology > TP Chemical technology > TP155 Chemical engineering
Kulliyyahs/Centres/Divisions/Institutes: Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Dr. Ma'an Alkhatib
Date Deposited: 12 Dec 2011 22:36
Last Modified: 06 Jul 2012 15:09
URI: http://irep.iium.edu.my/id/eprint/9230

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