In-vivo antiparasitic assessment of allicin against the growth and survival of haemoflagellate, Trypanosoma evansi

Baba, Mohd Shukri and Abu Hassan, Zainal Abidin and Latip, Jalifah (2016) In-vivo antiparasitic assessment of allicin against the growth and survival of haemoflagellate, Trypanosoma evansi. In: Seminar Nasional Peranan Ubat Herbal Sebagai Antimikrobial 2016 (Sem-NAS), 22-23 April 2016, Yogjakarta Plaza Hotel, Yogjakarta Indonesia. (Unpublished)

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Abstract

Cell morphological changes are frequently used as indirect indicators of the effect of studied materials on targeted cells. In this study, antiparasitic effects of active compound, namely allisin or diallyl thiosulfinate (C3H5SS(O)C3H5) in which extracted from the garlic (Allium sativum) was compared with anti-trypanosomal drug berenil, on the growth and survival of the haemoflagellate protozoa Trypanosoma evansi in mice. Groups of male ICR strain mice aged 6 – 8 weeks with 20 – 25g body weight were infected with the parasite (5.0 × 103 T. evansi per mouse) and orally given pre-, concurrent- or post-infection treatments with allicin (0.1 ml of 15 µg/ml alisin per mouse). Stained blood slides were prepared and examined under the light and electron microscopes for the evaluation of specified parameters. The results showed that the mice in negative control group (untreated but infected mice) succumbed to the T. evansi infections with rapid increase of parasitaemias and survived in a short period of time. The survival time for the mice in pre-infection treatment groups was longer (96.58 ± 0.2 days) than those of the mice in the groups receiving concurrently or post-infection treatments. There was also a positive relationship (p ≤ 0.05, n = 6) between the mice survival time and the ability to inhibit the parasites growth in this group. The morphological changes of T. evansi cells were observed where the undulating membrane was destroyed and the cell became crescent-shaped. Finally, both of the posterior and anterior ends were tapered before the flagellum destroyed and disintegrated in which lead to death of the cells. The cell morphological changes in berenil-treated mice was occurred much earlier (2nd -3rd hour post-treatment) and totally disappeared from the blood circulation within 5-6 hour later. The destruction of these parasite cells allowed the mice survived more than 100 days. The results from this study suggest that allicin has a stronger anti-parasitic activity against T. evansi by causing the destruction of the cells. Further studies are required to elucidate the mechanism of action of allicin on these cell structures.

Item Type:	Conference or Workshop Item (Slide Presentation)
Subjects:	Q Science > QR Microbiology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011)
Depositing User:	Dr Mohd Shukri Baba
Date Deposited:	13 Sep 2021 11:32
Last Modified:	11 Oct 2021 14:34
URI:	http://irep.iium.edu.my/id/eprint/92193

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