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Sperm morphology and testis histological changes in 12% high cholesterol diet administered rats following Tualang honey supplementation and diet modifications

Hasan, Sakiinah and Abdul Rahim, Roslina and Alias, Mohd Afzal and Muhammad, Naznin and Abdullah, Nor Zamzila and Abdul Razak, Redzuan Nul Hakim (2021) Sperm morphology and testis histological changes in 12% high cholesterol diet administered rats following Tualang honey supplementation and diet modifications. In: MSPP 34th Scientific Meeting 2021: Trailblazing The Translational Research, Monash University Malaysia (Virtual Meeting). (Unpublished)

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Abstract

Background: Hypercholesterolaemia is recognized as a factor associated with male infertility. However, currently, there is limited therapy available. Tualang honey (TH) is a type of Malaysian polyfloral wild honey produced by the rock bee (Apis dorsata) proven to exert both anti-inflammatory and anti-oxidative effects. Objective: This study aimed to determine the effects of TH coupled with diet modification on sperm morphology and testis histology of 12% high cholesterol diet (HCD) administered rats. Methodology: Twenty rats were divided into 4 groups. Control group were fed with standard diet for 20 weeks. The other 3 groups were fed with 12%HCD for 16 weeks. Then, group A rats were not treated and continued HCD. Group B rats were supplemented with TH (3.0g/kg) and continued HCD, while group C was given TH (3.0g/kg) but was changed to a normal diet for 4 weeks. At the end of 20 weeks, the left testis was collected for histology analysis and the cauda epididymis was collected to analyse the sperm parameter. Results: Compared to control, group A showed significant decrease in the percentage of normal sperm morphology (p<0.001). In contrast, group B and C demonstrated significant improvement in sperm morphology compared to group A (p<0.001). Group C showed better improvement in the percentage of normal sperm morphology compared to group B although not significant (p>0.05). However, there are no significant changes in Johnsen testicular histology scoring between the groups. Conclusion: Diet modification in addition to TH supplementation may further improve male fertility in HCD rats. Based on our findings, there is a need to further explore the potential TH in improving male infertility associated with hypercholesterolaemia.

Item Type: Conference or Workshop Item (Poster)
Additional Information: 5456 / 91806
Uncontrolled Keywords: High cholesterol diet, Tualang honey, sperm morphology, testis histology, Johnsen testicular scoring
Subjects: Q Science > QP Physiology
R Medicine > R Medicine (General)
R Medicine > RB Pathology
R Medicine > RC Internal medicine
R Medicine > RC Internal medicine > RC627 Specialties of Internal Medicine-Metabolic Diseases
R Medicine > RG Gynecology and obstetrics
R Medicine > RZ Other systems of medicine
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Medicine
Kulliyyah of Medicine > Department of Basic Medical
Kulliyyah of Medicine > Department of Pathology & Lab Medicine
Kulliyyah of Nursing
Kulliyyah of Nursing > Department of Basic Medical Sciences for Nursing
Depositing User: Dr ROSLINA ABDUL RAHIM
Date Deposited: 23 Sep 2021 10:25
Last Modified: 23 Sep 2021 10:25
URI: http://irep.iium.edu.my/id/eprint/91806

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