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Comparison of Ex-Situ and In-Situ transesterification for the production of microbial biodiesel

Hazmi, Alia Tasnim and Ahmad, Farah and Athoillah, Ahdyat Zain and Ahmad Tariq, Jameel (2021) Comparison of Ex-Situ and In-Situ transesterification for the production of microbial biodiesel. Bulletin of Chemical Reaction Engineering & Catalysis. ISSN 1978-2993

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Abstract

Microbial biodiesel is converted from microbial lipids via transesterification process. Most microbial biodiesel re-search is focusing on the use of microalgal lipids as feedstock. Apart from the microalgae, lipids can also be ex-tracted from other oleaginous microorganisms like fungi and yeast. However, there are gaps in the studies of lipid production from filamentous fungi, especially in-situ transesterification process. The aim of this project is to com-pare in-situ with the ex-situ transesterification of fungal biomass from Aspergillus oryzae. In ex-situ transesterifi-cation, two methods of lipid extraction, Soxhlet extraction and Bligh and Dyer extraction, were performed. For in-situ transesterification, two methods using different catalysts were investigated. Base-catalyzed in-situ trans-esterification of fungal biomass resulted on the highest Fatty Acid Methyl Esters (FAME) yield. The base-catalyzed in-situ transesterification was further optimized via Central Composite Design (CCD) of Response Sur-face Methodology (RSM). The parameters investigated were the catalyst loading, methanol to biomass ratio and reaction time. The optimization showed that the highest FAME yield was at 25.1% (w/w) with 10 minutes reaction time, 5% catalyst and 360:1 of the ratio of the methanol to biomass. Based on Analysis of Variance (ANOVA), the model was found to be significant according to the value of “Prob >F” of 0.0028

Item Type: Article (Journal)
Additional Information: 7967/91663
Uncontrolled Keywords: Aspergillus; Biodiesel; Ex-situ transesterification; In-situ transesterification; Lipid; Solvent extraction
Subjects: T Technology > TP Chemical technology > TP155 Chemical engineering
T Technology > TP Chemical technology > TP248.13 Biotechnology
T Technology > TP Chemical technology > TP315 Fuel
T Technology > TP Chemical technology > TP500 Fermentation industries. Beverages. Alcohol
T Technology > TP Chemical technology > TP670 Oils, fats, waxes
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering
Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Dr Farah Binti Ahmad
Date Deposited: 24 Aug 2021 16:29
Last Modified: 24 Aug 2021 16:29
URI: http://irep.iium.edu.my/id/eprint/91663

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