Step by step preparation of urine samples for in-Solution, gel-free proteomic studies, suitable for MALDI TOF MS and LCMS QTOF MS

Pakeer, Aniza and Idorus, Mohd Yusri and Ismail, Nosrihah and Mariappan, Vanitha Mariappan and Mustafa, Imad Al-Deen (2021) Step by step preparation of urine samples for in-Solution, gel-free proteomic studies, suitable for MALDI TOF MS and LCMS QTOF MS. In: 4th Kuantan Research Day 2020, Virtual.

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Abstract

Two-dimensional electrophoresis is an established method for isolating and separating proteins for proteomic analysis and identification of proteins. However, it requires a lot of optimization and it suffers from some ongoing concerns regarding quantitative reproducibility and limitations on its ability to study certain class of proteins. We propose a simpler method in preparing urine samples from acute melioidosis patients for in-solution proteomics using liquid chromatography coupled with mass spectrometry quadrupole time of flight (LCMS-QTOF MS) or matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). This method is relatively easier than 2-dimensional electrophoresis, affordable, reproducible, can be performed in less equipped laboratories for transport to specialized centers for proteomic studies and bioinformatic analysis.

Item Type:	Conference or Workshop Item (Other)
Uncontrolled Keywords:	proteomics, in-solution, LCMS, urine , melioidosis, proteome
Subjects:	R Medicine > R Medicine (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Medicine > Department of Basic Medical
Depositing User:	Mrs Norsyafini Ahmad Marzuki
Date Deposited:	09 Aug 2021 15:27
Last Modified:	09 Aug 2021 15:27
URI:	http://irep.iium.edu.my/id/eprint/90832

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