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Production of biosurfactants and bioemulsifiers by indigenous bacteria isolated from petroleum sludge and their association with total petroleum hydrocarbon degradation

Yusof, Nur Amira and Annuar, Nadhrah and Mohd Ashaari, Mardiana (2019) Production of biosurfactants and bioemulsifiers by indigenous bacteria isolated from petroleum sludge and their association with total petroleum hydrocarbon degradation. In: International Congress of the Malaysian Society for Microbiology 2019, 11th-14th November 2019, Royal Chulan, Seremban. (Unpublished)

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Microbial biosurfactants and bioemulsifiers are amphiphilic, surface-active compounds produced during biodegradation, increasing the bioavailability of organic pollutants such as hydrocarbons. Both are known to be produced by bacteria to facilitate the process of hydrocarbon degradation and at the same time enhancing oil recovery in petroleum industry. Therefore, the existence of indigenous microorganisms that have the ability to consume petroleum hydrocarbon as carbon source and simultaneously produce biosurfactants and bioemulsifiers in order to facilitate hydrocarbon metabolism can be manipulated for bioremediation purposes. This study aimed to determine the association between the ability of indigenous bacteria isolated from petroleum sludge to produce biosurfactants and bioemulsifiers with their ability to degrade total petroleum hydrocarbons (TPH). Preliminary screenings of biosurfactant (i.e surface tension measurement) and bioemulsifier activity (i.e emulsification assay) from the total number of 26 isolates revealed some potential biosurfactant-producing bacteria (BSP) while some were potentially bioemulsifier-producing bacteria (BEP). Highest emulsification index (E24) exhibited by potential BEP (≈ 72.81 %) while for the rest of the isolates were between 64 to 68 %. Surface tension measurement revealed the biosurfactant activity of the isolates, which were as low as ≈ 18.92 mN/m for a potential BSP; while the rest were between the range of 45 to 28 mN/m. Two of these bacterial isolates (one potential BSP and one potential BEP) were further investigated for TPH biodegradation study using GC-MS. Assuming growth-linked biodegradation; growth curves of these bacterial isolates showed acclimation period for up to more than 72 hours of incubation; whereby no evident disappearance of TPH had been detected. However, after six days, rate of TPH loss became rapid; whereby biodegradation percentage of TPH was about (≈ 73 %). By the twelfth day of biodegradation study, the percentage of TPH loss was up to (≈ 85 %). In conclusion, both potential BSP and BEP were potent hydrocarbon biodegraders and that both biosurfactants and bioemulsifiers were unique microbial products showing advantageous features in hydrocarbon biodegradation. Both bioemulsifiers and biosurfactants could be extracted and purified from bacteria as both molecules have great potential for application in green technology.

Item Type: Conference or Workshop Item (Poster)
Subjects: Q Science > QR Microbiology
T Technology > TD Environmental technology. Sanitary engineering > TD172 Environmental pollution
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Science > Department of Biotechnology
Depositing User: Dr. Mardiana Mohd. Ashaari
Date Deposited: 06 Jul 2021 16:49
Last Modified: 06 Jul 2021 16:49
URI: http://irep.iium.edu.my/id/eprint/90584

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