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Profiling of compounds in hydrosol extract of Aquilaria (Agarwood) species using gas chromatography-mass spectrometry (GCMS)

Hashim, Yumi Zuhanis Has-Yun and Jafar Ali, Natasha and Zainurin, Nur Aimi Aliah and Abbas, Phirdaous (2021) Profiling of compounds in hydrosol extract of Aquilaria (Agarwood) species using gas chromatography-mass spectrometry (GCMS). Biological and Natural Resources Engineering Journal, 5 (1). pp. 25-33. E-ISSN 2637-0719

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Most research involving agarwood mainly focused on extracting the agarwood oil from the infiltrated resin, the most sought non-timber forest product from Aquilariaspecies with high demand and prices. This led to other parts of this valuable plant species being considered as waste. This includes the agarwood hydrosol (by-product of water distillation). Hydrosol has been reported to retain certain aromatics of its primary essential oil. Therefore, the aim of the present study is to identify the composition of the agarwood hydrosol extracts from Aquilariaspecies by using gas chromatography-mass spectrometry (GCMS). Six agarwood hydrosol samples from different batches of production were used in this study and designated as S1 until S6. The hydrosol extracts were prepared in three steps; isolation of dissolved essential oil, filtration of the hydrosol extract and finally the evaporation of solvent (hexane). The resulting extracts were subjected to GCMS analysis. A total of 46, 48, 40, 53, 58 and 62 compounds were detected in hydrosol extract samples of S1, S2, S3, S4, S5 and S6, respectively. Accordingly, the oxygenated and phenolic compounds were found to be present in all hydrosol extract samples. The detected phenolic compounds include phenol, 2,5-bis (1,1-dimethylethyl)-,.phenol, 2,4-bis (1,1-dimethylethyl)- and 9-octadecenoic acid (Z)-, methyl ester. These compounds are believed to possess various potential biological activities including antioxidant activities. Sample S1 and S2 consist of sesquiterpene compounds such as (-)-aristolene, naphthalene, decahydro-4a-methyl-1- methylene-7- (1-methylethylidene)-,(4aR-trans), azulene,1,2,3,3a,4,5,6,7-octahydro-1,4-dimethyl-7- (1-methylethenyl)-,[1R-(1.alpha.,3a.beta.,4.alpha.,7.beta.)]- and alloaromadendrene where the later had the highest number of oxygenated and antioxidant compounds. In conclusion, results from this study suggests that agarwood hydrosol possesses myriad of compounds that warrant further investigation based on their potential medicinal benefits. This in turn could help realize the waste to wealth paradigm as well as promote the material as sustainable source for development of halal therapeutics.

Item Type: Article (Journal)
Uncontrolled Keywords: Agarwood, Aquilaria, Compounds, Gas chromatography-mass spectrometry, and Hydrosol.
Subjects: Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): International Institute for Halal Research and Training (INHART)
Kulliyyah of Engineering
Kulliyyah of Engineering > Department of Biotechnology Engineering
Date Deposited: 02 Jul 2021 08:12
Last Modified: 02 Jul 2021 08:12
URI: http://irep.iium.edu.my/id/eprint/90446

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